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CircRNA profiling in laminar shear stress-treated human umbilical vein endothelial cells.

ABSTRACT: CircRNA profiling in laminar shear stress-treated human umbilical vein endothelial cells.

PROVIDER: PRJNA950906 | ENA |

REPOSITORIES: ENA

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CircRNA profiling in laminar shear stress-treated human umbilical vein endothelial cells.

Project description:Laminar shear stress (LSS) suppresses endothelial inflammation and protects the arteries from atherosclerosis. Circular RNAs (circRNAs) are powerful regulators of vascular homeostasis and atherosclerosis; however, their roles in mediating the effects of LSS remain unexplored. To identify the changes in circRNA expression patterns after shear stress stimulation, we conducted circRNA microarray analysis using RNA extracted from HUVECs cultured for 24 h under static or LSS conditions.

2023-09-29 | GSE228636 | GEO

HUVECs cultured under normal or high laminar shear stress

Project description:Many studies have characterised the effect of normal laminar shear stress (LSS) on endothelial responses, however elevated shear stress, as would be experienced overlying a stenotic plaque, has not been studied in depth. Therefore we used transcriptomics and related functional analyses to compare cells exposed to laminar shear stress at 15 or 75 dynes/cm2 for 24 hours (LSS15-normal or LSS75-high shear stress). Human umbilical vein endothelial cells (HUVEC n=4 per flow condition from different batches of pooled donor HUVEC p2) were cultured for 24 hours on gelatin coated slides under laminar shear stress of either 15 dynes/cm2 or 75 dynes/cm2 (LSS15 or LSS75) to assess the effect of high shear stress on endothelial cells. Total RNA was obtained using Qiagen kit and array analysis performed by Service XS (Leiden, Netherlands).

2011-02-01 | E-GEOD-23289 | biostudies-arrayexpress

Transcription profiling by array of human young and senescent HUVECs under static and laminar shear stress conditions

Project description:Laminar shear stress due to constant blood flow is known to play a critical role in maintaining vascular health. In contrast, endothelial cell senescence appears to be closely associated with the incidence of vascular disorder. In an attempt to identify functional biomarkers for age-related vascular health/disease, the present study investigated differential gene expression of young and senescent human umbilical vein endothelial cells (HUVECs) under static and laminar shear stress. We used a cDNA microarray method to compare gene expression profiles of young and senescent HUVECs under static and laminar shear stress conditions. Experiment Overall Design: Senescent cells were prepared by continuous subculture in vitro, and a cone-and-plate device was used to impose laminar shear stress onto cells. Young and senescent cells were exposed to laminar shear stress or maintained under static conditions. Total mRNA was extracted and gene expression profiles were analyzed by cDNA microarray.

2009-06-01 | E-GEOD-13712 | biostudies-arrayexpress

Differential gene expression of young and senescent HUVECs under static and laminar shear stress conditions

2009-05-23 | GSE13712 | GEO

Proteomic Evaluation of the Influence of Shear Stress and BRD4-Inhibition on Human Umbilical Vein Endothelial Cells

Project description:Atherosclerosis is an important pathological factor in the development of cardiovascular diseases. In addition to increased plasma lipid concentrations, irregular/oscillatory shear stress and inflammatory processes trigger pathophysiological changes. Inhibitors of the transcription modulatory bromo- and extra-terminal domain (BET) protein family (BETi) could offer a possible therapeutic approach due to their anti-inflammatory properties. In this study, the influence of laminar shear stress, inflammation and BETi on human endothelial cells in an atherosclerosis in vitro model was investigated using global protein expression profiling. For this purpose, human umbilical cord derived vascular endothelial cells (HUVEC) were treated with TNFα to mimic the inflammatory condition and were exposed to 24h laminar shear stress in the presence or absence of a BRD4 inhibitor, JQ1. Data-independent acquisition mass spectrometry (DIA-MS) alloqed us to quantify 3316 proteins for further statistical analysis. Differentially regulated proteins indicate a clear influence of inflammation and shear stress on human endothelial cells. Overall, application of JQ1 is led to significant changes in the proteome, including a strong anti-inflammatory response as well as a potentially negative impact on atherosclerosis formation. To our knowledge, this is the first proteomics study on HUVEC which investigates the influence of shear stress and BET inhibition in TNFα inflammatory endothelial cell culture model.

2022-10-30 | PXD035842 | Pride

HUVECs cultured under normal or high laminar shear stress

2011-02-01 | GSE23289 | GEO

Sex-specific transcriptional differences between male and female human umbilical vein endothelial cells (HUVEC) after shear stress.

Project description:To assess whether there is a sex-specific response to stress, we subjected male and female HUVEC to laminar shear of 6 dyn/cm2 for 24 hours and analysed changes in gene expression. 6.7 % of all mRNAs were regulated by shear stress. Female HUVEC showed a more pronounced transcriptional response to shear than did their male counterparts. In addition to quantitative differences, a number of genes were regulated in the opposite direction between the two sexes by shear stress.

2014-12-31 | GSE52211 | GEO

mRNA microarray related to circRNA in human umbilical vein endothelial cells

Project description:mRNA microarray related to circRNA in human umbilical vein endothelial cells

| PRJNA843948 | ENA

MicroRNA microarray related to circRNA in human umbilical vein endothelial cells

Project description:MicroRNA microarray related to circRNA in human umbilical vein endothelial cells

| PRJNA843949 | ENA

Genome-wide analysis of mechano-responsive gene expression of mesenchymal stem cells after laminar shear stress

Project description:Analysis of mechano-regulation of mesenchymal stem cell gene expression level. The hypothesis tested in the present study was that laminar shear stress influences the cytoskeleton arrangement and amount and then triggers seriers of cell metabolism and cellular functions. Results provide important information of the response of mesenchymal stem cell to laminar shear stress, such as specific mechano-responsive genes, up- or down-regulated specific anabolic/catabolic cellular functions.

2018-12-31 | GSE109180 | GEO

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