Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Multimodal characterization of murine gastruloid development

ABSTRACT: Multimodal characterization of murine gastruloid development

PROVIDER: PRJNA954338 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR24128849_1.fastq.gz	Fastqsanger.gz
	SRR24128849_2.fastq.gz	Fastqsanger.gz
	SRR24128850_1.fastq.gz	Fastqsanger.gz
	SRR24128850_2.fastq.gz	Fastqsanger.gz
	SRR24128851_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 36

Similar Datasets

Multimodal characterization of murine gastruloid development

Project description:Gastruloids are highly scalable, three-dimensional assemblies generated from pluripotent stem cells that recapitulate fundamental principles of embryonic pattern formation in vitro. Using single cell RNA and multiome sequencing we provide a comprehensive resource mapping cellular states and cell types found during gastruloid development and compare them to the in vivo embryo. We further develop a high throughput gastruloid handling and imaging pipeline to spatially monitor cell type emergence and unfolding of symmetry breaking during gastruloid development. We report spatial variability of pluripotency states in early gastruloids that determines a binary cell response to Wnt activation. While cells situated in the core of the gastruloid revert to an ectopic pluripotent state, peripheral cells differentiate to a primitive streak like state. These two populations then cause gastruloids to break radial symmetry, allowing axial elongation and commitment to the three embryonic germ layers. Finally by performing a phenotypic compound screen, we perturb thousands of gastruloids at relevant developmental time points deriving a phenotypic landscape and inferring molecular regulator networks underlying gastruloid development. Employing this resource, we improve the formation of anterior structures in the existing gastruloid model, using a dual Wnt modulation approach to differentiate an anterior ectopic pluripotent core to anterior ecto- and endodermal structures. This work gives is a resource to understand how gastruloids develop and, more generally, how homogenous cell populations can generate complex patterns in vitro.

2023-09-07 | GSE229386 | GEO

Multimodal characterization of murine gastruloid development.

Project description:Gastruloids are 3D structures generated from pluripotent stem cells recapitulating fundamental principles of embryonic pattern formation. Using single-cell genomic analysis, we provide a resource mapping cell states and types during gastruloid development and compare them with the in vivo embryo. We developed a high-throughput handling and imaging pipeline to spatially monitor symmetry breaking during gastruloid development and report an early spatial variability in pluripotency determining a binary response to Wnt activation. Although cells in the gastruloid-core revert to pluripotency, peripheral cells become primitive streak-like. These two populations subsequently break radial symmetry and initiate axial elongation. By performing a compound screen, perturbing thousands of gastruloids, we derive a phenotypic landscape and infer networks of genetic interactions. Finally, using a dual Wnt modulation, we improve the formation of anterior structures in the existing gastruloid model. This work provides a resource to understand how gastruloids develop and generate complex patterns in vitro.

| S-EPMC10241222 | biostudies-literature

Symmetry breaking in gastruloid development

Project description:Symmetry breaking in gastruloid development

| PRJNA759809 | ENA

Symmetry breaking in gastruloid development

Project description:Symmetry breaking in gastruloid development

| PRJNA954812 | ENA

Symmetry breaking in gastruloid development

Project description:We use single cell RNA sequencing to describe the transcriptional changes during gastruloid development from 0 to 120h hours.

2023-04-29 | GSE229513 | GEO

Symmetry breaking in gastruloid development

Project description:We use single cell RNA sequencing to describe the transcriptional changes during gastruloid development from 24 to 84 hours with 12 hours intervals.

2021-09-04 | GSE183255 | GEO

Single cell analysis of gastruloid hemogenic development

Project description:Time-course analysis of cell type specification in a 3-dimensional gastruloid differentiation system initiated from mouse embryonic stem (ES) cells and biased towards hemato-endothelial cell production.

2022-10-09 | E-MTAB-12148 | biostudies-arrayexpress

Single cell analysis of gastruloid hemogenic development

Project description:Single cell analysis of gastruloid hemogenic development

| PRJEB56021 | ENA

Characterization of dynamic p300 enhancers in murine cardiomyocyte development

Project description:Characterization of dynamic p300 enhancers in murine cardiomyocyte development

| PRJNA802575 | ENA

Characterization of dynamic p300 enhancers in murine cardiomyocyte development [HiC]

Project description:Characterization of dynamic p300 enhancers in murine cardiomyocyte development [HiC]

| PRJNA799015 | ENA