Project description:To evaluate the global signaling changes induced by Neuronal Regeneration Related Protein (NREP) downregulation, we employed antibody-microarrays in human primary hepatocytes lacking NREP.
Project description:To evaluate the global transcriptomic changes induced by Neuronal Regeneration Related Protein (NREP) downregulation, we employed RNA-sequencing in human primary hepatocytes lacking NREP.
Project description:Human primary hepatocytes isolated from chimeric mice were infected with HBV for 7 days. The comprehensive changes of miRNA levels were determined by miRNA array. MicroRNA expression levels were compared in between control and HBV-replicating primary hepatocytes by 2K microRNA microarrays
Project description:Mouse primary hepatocytes (MPH) prepared from C57Bl6 male adult mice were challenged with the FXR agonist GW4064 and used to prepare RNA which was then processed for analysis using MoGene-2_0-st Affymetrix microarrays according to standard procedures.
Project description:Liver gene expression was analyzed by microarrays at different times during 24h. This was done in mice fed a regular diet (Low fat diet: LFD) or a high fat diet (HFD). The experiment was done in wild-type mice (P110aHepWT) and in mice lacking p110alpha in hepatocytes (P110aHepKO).
Project description:Mouse primary hepatocytes (MPH) prepared from C57Bl6 male adult mice were treated with the ERS-drug thapsigargin and ChIP-seq was performed using an H3K27ac antibody (Active Motif ref 39685).
Project description:Previously published data suggested some redundant functions between HDAC1 and HDAC2 in mouse. To test this hypothesis, we used microarrays to have a genome wide analysis at the transcription level of primary MEFs lacking HDAC1, HDAC2.