Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MLX siRNA knockdown in primary human hepatocytes

ABSTRACT: MLX siRNA knockdown in primary human hepatocytes

PROVIDER: PRJNA907289 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR22480125.fastq.gz	Fastqsanger.gz
	SRR22480126.fastq.gz	Fastqsanger.gz
	SRR22480127.fastq.gz	Fastqsanger.gz
	SRR22480128.fastq.gz	Fastqsanger.gz
	SRR22480129.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 29

Similar Datasets

MLX siRNA knockdown in primary human hepatocytes

Project description:3' mRNA-sequencing of primary human hepatocytes treated with either non-targeting (siNT) or targeted siRNA against MLX (siMLX) (3 independent replicates with different PHH donors, n=5 prepared per donor and treatment; one siMLX sample was excluded from analysis due to low quality)

2023-04-21 | GSE219162 | GEO

RNAseq analysis of ClpP siRNA treated mouse primary hepatocytes

Project description:We report the gene expression profile in mouse primary hepatocytes after treatment of ClpP siRNA.

2024-07-21 | GSE180590 | GEO

RNAseq analysis of ClpP siRNA treated mouse primary hepatocytes

Project description:RNAseq analysis of ClpP siRNA treated mouse primary hepatocytes

| PRJNA748748 | ENA

MLX transcriptional regulation in muscle cells

Project description:This study set out to identify MLX transcriptional targets in muscle cells. C2C12 Myoblasts were virally transduced to increase MLX activity, by overexpression of the wild-type protein; and to decrease MLX activity by overexpression of a dominant negative MLX protein and by shRNA induced knockdown of MLX. Transcripts that were significantly and consistently regulated by the different modes of MLX modulation were identified. The largest proportion of these were genes encoding secreted proteins including growth factors, cytokines and extracellular proteins. We therefore conclude that MLX can regulate myokine transcripts. mRNA profiles from C2C12 muscle cells with increased and decreased MLX activity were examined.

2016-01-04 | E-GEOD-70028 | biostudies-arrayexpress

siRNA-mediated knockdown

Project description:siRNA-mediated knockdown

| PRJNA541912 | ENA

MLX transcriptional regulation in muscle cells

2016-01-04 | GSE70028 | GEO

Transcriptional and genomic occupancy profiling from MLX WT and MLX knockout mouse tissues.

Project description:We generated mice null for MAX-like Protein X (MLX), encoded by Mlx. All male mice are sterile. We profiled testes tissue from WT versus KO mice by RNA-Seq. We performed ChIP-Seq on WT and KO testes for MLX and MAX, as well as ChIP-Seq for MLX and MAX from primary B220+ splenic B cells, and ChIP-Seq for MLX, MAX and MNT from 3T3 cell lines derived from WT and KO embryos.

2021-07-31 | GSE165820 | GEO

Coordinated Cross-Talk Between the Myc and Mlx Networks in Liver Regeneration and Neoplasia

Project description:The Myc bHLH-ZIP transcription factor is deregulated by most cancers. As a heterodimer with the bHLH-ZIP protein Max, Myc regulates target genes that contribute to metabolism and proliferation. This “Myc Network” cross-talks with the “Mlx Network” comprised of the Myc-like bHLH-ZIP proteins MondoA and ChREBP and the Max-like bHLH-ZIP protein Mlx. This “Extended Myc Network” regulates genes with both common and distinct functions. We have generated hepatocytes lacking Mlx (mlxKO) or Mlx+Myc (double KO or DKO) and quantified their abilities to replace dying hepatocytes in a murine model of Type I tyosinemia. We find that this function deteriorates as the Extended Myc Network is progressively dismantled. Genes dysregulated in mlxKO and DKO hepatocytes include those involved in translation and mitochondrial function. The Myc and Mlx Networks thus cross-talk with the latter playing a disproportionate role. mycKO and mlxKO mice also develop age-dependent non-alcoholic fatty liver disease and mlxKO and DKO mice develop extensive hepatic adenomatosis not observed in wild-type, mycKO, chrebpKO or mycKOxchrebpKO mice. In addition to demonstrating cooperation between the Myc and Mlx Networks, this study reveals the latter to be more important in maintaining metabolic and translational homeostasis, while concurrently serving as a suppressor of benign tumorigenesis.

2022-05-05 | GSE181371 | GEO

miR1908-5p primary hepatocytes

Project description:miR1908-5p primary hepatocytes

| PRJNA745934 | ENA

Super-enhancer-driven MLX mediates redox balance maintanance via SLC7A11 in osteosarcoma

Project description:A systematic analysis of super-enhancers identified MLX as a potential oncogene in osteosarcoma. Knockdown of MLX impaired tumor aggressiveness in vitro and in vivo, suggesting oncogenic properties of MLX. Mechanistically, silencing of MLX downregulates SLC7A11, a key gene encoding glutamate/cystine antiporter, to attenuate the uptake of cystine and interrupt the redox balance, leading to ferroptotic cell death. Pharmacological inhibition of SLC7A11 triggered massive ferroptosis and caused impaired tumor growth, providing a promising approach for osteosarcoma treatment.

2023-08-08 | GSE220173 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data