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Solanum lycopersicum

ABSTRACT: Solanum lycopersicum Genome sequencing and assembly

PROVIDER: PRJNA954457 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR24164314_1.fastq.gz	Fastqsanger.gz
	SRR24164314_2.fastq.gz	Fastqsanger.gz
	SRR24164315_1.fastq.gz	Fastqsanger.gz
	SRR24164315_2.fastq.gz	Fastqsanger.gz
	SRR24164316_1.fastq.gz	Fastqsanger.gz

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microRNA-target RNA pairs revealed by Parallel analysis of RNA ends in Solanum lycopersicum

Project description:Solanum lycopersicum RNA degradome sequencing Isolated polyadenylated RNA from total RNA extracts of Solanum lycopersicum, were ligated to 5'-adapter that includes an MmeI recognition site. The ligated products were purified again, reverse transcribed and cleaved with MmeI. The 5' fragments were purified from gel and ligated to a 3'- dsDNA adapter and PCR amplified. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

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| PRJNA1109536 | ENA

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