Project description:There is an urgent need for early detection of Pancreatic ductal adenocarcinoma (PDAC). Due to high stability and tight relationship with tumor genesis and development, miRNA has been considered as a type of potential biomarkers for PDAC detection and surveillance. However, up to now, the common miRNA panel for PDAC detection is still undetermined. In order to reveal the differential miRNA expression profiles between PDAC patients(P group), chronic pancreatitis patients(C group) and healthy persons(H group), plasma samples were collected from threegroups, further the genome wide small RNA expression profiling was performed.
Project description:The purpose of our study was to identify miRNAs that can predict islet transplantation outcomes. We hypothesize that miRNAs may be used to predict islet function post-transplantation in TPIAT patients
Project description:Plasma miRNA expression profiling of pancreatic ductal adenocarcinoma patient, chronic pancreatitis patients and healthy population in China
Project description:Autoimmune pancreatitis (AIP) is a disease with unclear immunologic triggers. This study shows that the pancreatic stellate cells(PSCs) are involved in the regulation of the immune response and can cause autoimmunity when the NF-κB signalling in these cells is disrupted. The PSCs were isolated from animals which show autoimmune pancreatitis (NEMO knockout group) or chronic pancreatitis (NEMO wildtype group).
Project description:Autoimmune pancreatitis (AIP) is a recently identified disease of the pancreas with unknown etiology and antigens. The aim of this study was to determine new target antigens and differentially regulated genes and proteins by means of transcriptomics and proteomics and to validate them in patients with autoimmune pancreatitis. Here we report a distinct downregulation at the RNA and protein level of pancreatic proteases (anionic trypsinogen, cationic trypsinogen, mesotrypsinogen, elastase IIIB) and pancreatic stone protein in autoimmune pancreatitis in comparison to alcohol-induced chronic pancreatitis.
Project description:Microarray was used to find out the differentially expressed miRNAs in Alcoholic Chronic Pancreatitis as compared to healthy control. Resulting miRNAs could be further used to understand the disease as well as could act as secretory biomarker.
Project description:Age-standardized incidence rates for pancreatic cancer (PC) in men have increased by 25% from 1957 to 2011 in Finland. The average age of diagnosis for PC is 69 years in Nordic males, whereas the average age of diagnosis of chronic pancreatitis (CP) is 40-50 years, but the cases overlap in age. By radiology the evaluation of a pancreatic mass, i.e. the differential diagnosis between CP and PC is often difficult. Preoperative needle biopsies are difficult to obtain and are demanding to interpret. New blood based biomarkers are needed. The accuracy of the only established biomarker for PC, CA 19-9 is rather poor in differentiating between benign and malignant mass of the pancreas. In this study, we have performed mass spectrometry HDMSE analysis of serum samples from patients with chronic pancreatitis and pancreatic cancer. We have quantified 652 proteins and performed detailed statistical analysis such as principal component analysis, orthogonal partial least square discriminant analysis and receiver operating curve analysis.
Project description:The roles of protein arginine methylation in the pathongenesis of chronic pancreatitis still remains poorly understood. In this study, we performed a proteomic identification of ADMA (asymmetric dimethylarginine)-containing proteins in clinical tissues collected from chronic pancreatitis patients using affinity purification combined with LC-MS/MS.
Project description:Autoimmune pancreatitis (AIP) is a disease with unclear immunologic triggers. This study shows that the pancreatic stellate cells are involved in the regulation of the immune response and can cause autoimmunity when the NF-κB signalling in these cells is disrupted.