Xenopus laevis
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ABSTRACT:
PROVIDER: PRJNA95677 | ENA |
REPOSITORIES: ENA
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Project description:Xenopus laevis tadpoles are capable of limb regeneration following amputation, in a process which initially involves the formation of a blastema. However, Xenopus has full regenerative capacity only through premetamorphic stages. We have used the Affymetrix Xenopus laevis Genome Genechip® microarray to perform a large-scale screen of gene expression in the regeneration-complete, stage 53 (st53), and regeneration-incomplete, stage 57 (st57), hindlimbs at 1 and 5 days post-amputation. Through an exhaustive reannotation of the Genechip® and a variety of comparative bioinformatic analyses, we have identified genes that are differentially expressed between the regeneration-complete and âincomplete stages, detected the transcriptional changes associated with the regenerating blastema, and compared these results with those of other regeneration researchers. We focus particular attention on striking transcriptional activity observed in genes associated with patterning, stress response, and inflammation. Overall, this work provides the most comprehensive views yet of a regenerating limb and different transcriptional compositions of regeneration-competent and deficient tissues. Experiment Overall Design: This experiment measures gene expression in regeneration competent stage 53 and regeneration non-competent stage 57 Xenopus laevis hindlimbs, at 1 and 5 days post-amputation. Four replicates for each condition were used. Hindlimbs at either st53 or st57 were amputated unilaterally or bilaterally at the mid-zeugopodia level. One (1dPA) and 5 days (5dPA) after amputation tissues were collected 1mm proximal to the original level of amputation. Total RNA was isolated using RNaqueous micro kit (Ambion, inc.). One microgram of total RNA was amplified with the the Affymetrix 2 cycle kit and assayed per Genechip using standard Affymetrix protocols.
2008-06-13 | E-GEOD-4738 | biostudies-arrayexpress
Project description:Xenopus laevis tadpoles are capable of limb regeneration following amputation, in a process which initially involves the formation of a blastema. However, Xenopus has full regenerative capacity only through premetamorphic stages. We have used the Affymetrix Xenopus laevis Genome Genechip® microarray to perform a large-scale screen of gene expression in the regeneration-complete, stage 53 (st53), and regeneration-incomplete, stage 57 (st57), hindlimbs at 1 and 5 days post-amputation. Through an exhaustive reannotation of the Genechip® and a variety of comparative bioinformatic analyses, we have identified genes that are differentially expressed between the regeneration-complete and –incomplete stages, detected the transcriptional changes associated with the regenerating blastema, and compared these results with those of other regeneration researchers. We focus particular attention on striking transcriptional activity observed in genes associated with patterning, stress response, and inflammation. Overall, this work provides the most comprehensive views yet of a regenerating limb and different transcriptional compositions of regeneration-competent and deficient tissues. Keywords: Expression profiling
2006-08-11 | GSE4738 | GEO
Project description:Analysis of whole body of unfertilized eggs and two-cell stage, 16-cell stage, stage 8, stage 9, stage 10.5, stage 12, stage 15, stage 20, stage 25, stage 30, stage 35 and stage 40 embryos. Results provide insight into the global molecular changes in Xenopus embryogenesis.
2015-09-05 | GSE40620 | GEO
Project description:ChIP-sequencing in stage 10.5 Xenopus laevis embryos
| PRJNA306102 | ENA
Project description:Xenopus laevis tadpoles differ in their regenerative potential according to their developmental stage. Here, we focus on tail regeneration following amputation. By comparing the regenerative response during the naturally occurring regeneration-competent and -incompetent stages, scRNAseq can reveal cell type changes that are required for successful regeneration.
2019-04-23 | E-MTAB-7716 | biostudies-arrayexpress
Project description:Developmental gene expression of Xenopus laevis J strain [stage]
| PRJNA296953 | ENA
Project description:Analysis of whole body of unfertilized eggs and two-cell stage, 16-cell stage, stage 8, stage 9, stage 10.5, stage 12, stage 15, stage 20, stage 25, stage 30, stage 35 and stage 40 embryos. Results provide insight into the global molecular changes in Xenopus embryogenesis. Gene expressions in intact whole unfertilized eggs and whole embryos at two-cell stage, 16-cell stage, stage 8, stage 9, stage 10.5, stage 12, stage 15, stage 20, stage 25, stage 30, stage 35 and stage 40.
2015-09-05 | E-GEOD-40620 | biostudies-arrayexpress
Project description:Xenopus laevis tadpoles display a decreasing capacity to regenerate their limbs following injury according to developmental stage. By comparing the regenerative response during the naturally occurring regeneration-competent, -restricted and -incompetent stages, scRNAseq can reveal cell type changes that are required for successful regeneration.
2021-05-17 | E-MTAB-9104 | biostudies-arrayexpress
Project description:Global analysis of the transcriptional network controlling Xenopus endoderm formation
| PRJNA94719 | ENA
Project description:Global analysis of RAR-responsive genes in the Xenopus neurula
| PRJNA91671 | ENA