Project description:Purpose: To understand the molecular mechanism between PNET2 and NTLs, we generated 35S:NTL-SRDX in pnet2_ab mutants to suppress NTL gene expression and performed the whole genome transcriptome analysis on 4-week-old plants of WT, pnet2_ab, 35S:NTL6-SRDX pnet2_ab, 35S:NTL9-SRDX pnet2_ab, and 35S:NTL12-SRDX pnet2_ab. Conclusions: PNET2 regulates diverse responses by associating with a family of membrane-bound transcriptional factors.
Project description:Transcriptional profiling of Arabidopsis transgenic 35S:AtMYB102-SRDX plant under MS medium containing 200mM NaCl for 24h. Transgenic Arabidopsis (background : Col-0) overexpressing AtMYB102 fused to an active transcriptional repression domain (SRDX) showed strong salt tolerance.
Project description:Using whole genome bisulfite sequencing to provide single-base resulution of DNA methylation status in 35S-SUC WT, hdp1-1 and hdp2-1, mbd7, idm1, and hdp2mbd7 double mutants
Project description:Using MethylC-Seq to provide single-base resolution of DNA methylation status in 35S-SUC2 WT and anti-silencing mutants( arp6-5, pie1-7, h2a.z-2, idm1-9 and ros1-14）
Project description:Arabidopsis emf2 mutants bypass vegetative development and flowering upon seed germination. We introduced a broccoli BoEMF2.1 gene into emf2 mutants and obtained rescued emf2 plants that harbored 35S::BoEMF2.1. We found that BoEMF2.1 can partially rescue the phenotype of emf2 to that of WT. We used microarrays to study the global program of gene expression and to identify genes misexpressed in the Arabidopsis emf2 mutant that had been rescued by 35S::BoEMF2.1.
Project description:6 days old Arabidopsis thaliana of Col-0 wild-type and 35S:ARR1-SRDX transgenic seedlings grown in liquid culture (1/2 MS, 1 g/L sucrose, 0.5 g/L MES, pH 5.7) were induced with either 5 µM 6-Benzyladenine (a cytokinin analog) for 15 or 120 min, or mock-treated 120 min as a control. These samples were subjected to microarray analysis.
Project description:Characterization of the impact of TT2 or MYB5 overexpression on gene expression. Col 0 plants were compared to 35S::TT2 and 35S::MYB5 overexpressors in control conditions
Project description:Regulation of stomatal movement is one of the effective strategies for developing resistant crops to air pollutant because stomata allows absorption of various air pollutants, such as ozone and sulfur dioxide. Transcription factor (TF) is a fascinating target of genetic manipulation for this end because TF regulates many genes simultaneously and methods of genetic manipulation are universally established. Here, we have screened transgenic Arabidopsis lines expressing chimeric repressor of TFs in high-concentration of ozone and found that the chimeric repressors of GOLDEN-LIKE1 (GLK1) and GLK2 (GLK1-SRDX and GLK2-SRDX) conferred strong tolerance to ozone. These 35S:GLK1/2-SRDX plants also showed sulfur dioxide tolerance. Their leaves showed lower rate of transpiration than wild type and a remarkable closed-stomata phenotype. The expression of the genes encoding K+ and water channels, including KAT1 and AKT1, which are involved in stomatal opening, was downregulated in 35S:GLK1-SRDX plants. Consistently, expression of GLK1-SRDX driven by the GC1 promoter, which has an activity only in guard cell, also induced closed-stomata and an ozone tolerant phenotype. On the contrary, 35S:GLK1/2 plants showed hypersensitivity to ozone and an opened-stomata phenotype. These data suggested that GLK1 and GLK2 have an ability to induce transcriptional change in guard cell and regulate stomatal movement. Our findings provide an effective tool to confer resistance to air pollutant by regulating stomatal aperture and improve crop productivity in future.
