Project description:RamA, a 113-amino-acid regulatory protein, belongs to the AraC-XylS transcriptional activator family. Here, the network of genes regulated by RamA was investigated by determining changes in transcript profiles when ramA gene was knocked out from wild type LTL strain, which expresses ramA constitutively.
Project description:We report the use of Illumina based transcriptome sequencing to determine the RamA Regulon in K. pneumoniae. This study has identified both genes and small RNAs that are upregulated in the presence of RamA.
Project description:We report the use of Illumina based transcriptome sequencing to determine the RamA Regulon in K. pneumoniae. This study has identified both genes and small RNAs that are upregulated in the presence of RamA. Comparison of genetically modified mutants; TS67 (delta ramA) and TS68 (delta ramR, ramA overexpresser)
Project description:RamA, a 113-amino-acid regulatory protein, belongs to the AraC-XylS transcriptional activator family. Here, the network of genes regulated by RamA was investigated by determining changes in transcript profiles when ramA gene was knocked out from wild type LTL strain, which expresses ramA constitutively. Analysis used aerobic log-phase state RNA from S. Typhimurium strain LTL (ciprofloxacin MIC: 4 µg/ml) derived from S. Typhimurium LT2 by in vitro-induction using ciprofloxacin as control samples for comparison to the experimental samples taken from its mutant strain, LTLvramA with ramA-deletion. Six biological replicates were used for the experiment.Two replicates for each sample. One replicate per array.
