Project description:Members of the tomato clade exhibit wide diversity in fruit coloration, growth habit, leaf morphology and mating preferences. However, the mechanisms governing inter-species diversity in fruit coloration are largely unknown. Therefore, a proteomic approach combined with carotenoid profiling and carotenogenic gene expression was used to decipher the diversity in carotenogenesis in green-fruited Solanum habrochaites, orange-fruited S. galapagense, and red-fruited S. pimpinellifolium with S. lycopersicum, cv. Ailsa Craig (tomato).
Project description:First expansion of the public Tomato brown rugose fruit virus (ToBRFV) Nextstrain build inclusion of new genomic and epidemiological data
Project description:To evaluate the role of seeds in fruit quality, we induced parthenocarpy in tomato by regulating ovule-specific auxin synthesis or responsiveness using the INO promoter from A. thaliana, which is expressed in the outer layer of the integuments during early stages of ovule development. We compared these to fruit where the same coding regions were expressed from the DeFH9 promoter which is expressed in carpel tissues during early stages of ovule development. Expression of auxin synthesis or responsiveness genes by both of these promoters produced seedless parthenocarpic tomato fruit. We compared fruit samples using the Affymetrix tomato GeneChip (GPL4741) to determine how gene regulation and expression differed between wild-type and transgenic fruit. Keywords: genetic modification
Project description:The environmental conditions greatly influence tomato fruit quality, by affecting the expression of genes, the abundance of metabolites and the perception of sensorial attributes.In this study, a fruit transcriptome investigation, together with a sensory test and a metabolomic analysis were per-formed to evaluate the impact of the environment on two popular tomato cultivars grown in two Italian regions. The transcriptional profile of each cultivar, cultivated in two different areas high-lighted differential expression in genes involved in pathways related to cell wall components such as pectin, lignin and hemicellulose, sugars as well as in amino acids, phenylpropanoids, and pigment synthesis. The cultivation area mainly affects sensory attributes related to texture and flavor and the metabolic pattern of cell wall precursors, sugars, glutamate, aspartate and carotenoids. In the two genotypes cultivated in the same environment, some attributes and fruit-related quality processes are similarly affected, while others are differently influenced based on tomato specific genetic makeup. Combination of transcriptomic, sensory and metabolomic data obtained from the two tomato genotypes revealed that the environment has a profound effect on specific sensory traits, providing information on factors that shape the specific characteristics and genetic targets for im-proving tomato fruit characteristics.
2024-09-13 | GSE273560 | GEO
Project description:EMG produced TPA metagenomics assembly of PRJEB36528 data set (Real-time tracking of Tomato brown rugose fruit virus (ToBRFV) outbreaks in the Netherlands using Nextstrain.).