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ABSTRACT: PacBio sequencing revealed the effects of different forms and application rates of silicon fertilizer on the composition of bacterial and fungal communities in sugarcane rhizosphere soil.

PROVIDER: PRJNA962000 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24308112.fastq.gz	Fastqsanger.gz
	SRR24308113.fastq.gz	Fastqsanger.gz
	SRR24308114.fastq.gz	Fastqsanger.gz
	SRR24308115.fastq.gz	Fastqsanger.gz
	SRR24308116.fastq.gz	Fastqsanger.gz

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Project description:Soil bacterial gene sequence in silicon fertilizer application

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Project description:Changes of bacterial community composition in soil and different parts of sugarcane after silicon fertilizer treatment

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Influence of Cochliobolus miyabeanus and silicon on gene expression in rice leaves (Oryza sativa L.)

Project description:Although Cochliobolus miyabeanus is an important fungal leaf pathogen on rice plants worldwide, it is largely neglected by molecular plant phytopathologists. To shed new light on the molecular and genetic basis of the rice M-bM-^@M-^S C. miyabeanus interaction, we compared the transcriptome of rice leaves 12h post inoculation to uninfected leaves. Even though usable sources of resistance against brown spot disease caused by C. miyabeanus are scarce, silicon application emerges as a sustainable protection strategy. Many articles report the beneficial effect of silicon on brown spot resistance. however the underlying mechanisms remain largely unclear. The influence of silicon application on the transcriptome of healthy and infected rice leaves 12hpi was compared as well in an attempt to disentangle the modulation of silicon-induced brown spot resistance. Comparison between C. miyabeanus- and mock-infected rice leaves 12h post inoculation. This study consist of a 2 x 2 factorial design (infected and non-infected; untreated and silicon-treated) in three biological replicates.

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The effects of different chemical forms of iron fertilizer on cadium accumulation in dwarf Polish wheat are reflected in the differentially expressed genes

Project description:Purpose: To understand the effects of two different chemical forms of iron fertilizer on cadmium accumulation Methods:Cultivation and treatment for three weeks of dwarf Polish wheat seedlings by hydroponics, in triplicate, qRT–PCR validation was performed using TaqMan and SYBR Green assays Results: Iron fertilizer can effectively reduce cadmium concentration in plants Conclusions: Our study represents the different chemical forms of iron fertilizer have different mitigation effects on cadmium. The transcriptome gata showed that iron fertilizer have changed the cadimium metabolism

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Project description:fertilizer application

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Project description:Changes of fungal communities in fertilizer application soils

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Project description:Effect of Silicon Fertilizer Treatment on Transcriptome of Sugarcane Stem and Leaf of "165204" Variety

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Analysis of the extracellular proteome from Aspergillus nidulans growing in Avicel, sugarcane bagasse and sugarcane straw

Project description:Lytic polysaccharide monooxygenases (LPMOs) are oxidative enzymes found in viruses, archaea, bacteria as well as eukaryotes, such as fungi, algae and insects, actively contributing to the degradation of different polysaccharides. Analysis of the extracellular proteome (secretome) from Aspergillus nidulans growing in Avicel, sugarcane bagasse and sugarcane straw and analysed by LC-MS/MS in a LTQ Orbitrap Velos revealed that up to five LPMOs from family AA9 (AnLPMO9s), along with an AA3 cellobiose dehydrogenase (AnCDH1), are co-secreted upon growth on crystalline cellulose and lignocellulosic substrates, indicating their role in the degradation of plant cell wall components. Functional analysis revealed that the three main secreted LPMO9s (AnLPMO9C, AnLPMO9F and AnLPMO9G) correspond to cellulose- active enzymes with distinct regioselectivity. Deletion and overexpression studies confirmed that the abundantly secreted AnLPMO9F is a major component of the extracellular cellulolytic system, while AnLPMO9G, less abundant in the secretome, and has an important role by oxidizing crystalline fractions of cellulose. Single or double deletion of these AnLPMO9s partially impair fungal growth on sugarcane straw but not on crystalline cellulose, demonstrating the importance of LPMO9s for the saprophytic fungal lifestyle in the degradation of complex lignocellulosic substrates. Although the deletion of AnCDH1 slightly reduced the cellulolytic activity, it did not affect fungal growth indicating the existence of other electron donors to LPMOs. Additionally, double or triple knockouts of these enzymes had no accumulative deleterious effect on the cellulolytic activity nor on fungal growth, regardless of the deleted gene. Overexpression of AnLPMO9s in a cellulose-induced secretome background confirmed the importance and applicability of AnLPMO9G to improve lignocellulose saccharification.

2022-05-09 | PXD031886 | Pride

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