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The role of MEIOC at the transition from mitosis to meiosis during mammalian spermatogenesis [bulkRNAseq]

ABSTRACT: The role of MEIOC at the transition from mitosis to meiosis during mammalian spermatogenesis [bulkRNAseq]

PROVIDER: PRJNA962505 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24323021.fastq.gz	Fastqsanger.gz
	SRR24323022.fastq.gz	Fastqsanger.gz
	SRR24323023.fastq.gz	Fastqsanger.gz
	SRR24323024.fastq.gz	Fastqsanger.gz
	SRR24323025.fastq.gz	Fastqsanger.gz

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The role of MEIOC at the transition from mitosis to meiosis during mammalian spermatogenesis [bulkRNAseq]

Project description:We investigated how MEIOC affects testicular germ cells at the transition from mitosis to meiosis. We performed gene expression profiling analysis using data obtained from RNA-seq of preleptotene-enriched testes from wild-type and Meioc KO pups.

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The role of MEIOC at the transition from mitosis to meiosis during mammalian spermatogenesis [scRNA]

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The role of MEIOC at the transition from mitosis to meiosis during mammalian spermatogenesis

Project description:The role of MEIOC at the transition from mitosis to meiosis during mammalian spermatogenesis

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Project description:The role of MEIOC at the transition from mitosis to meiosis during mammalian spermatogenesis [scRNA]

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Poised chromatin and bivalent domains facilitate the mitosis-to-meiosis transition in the male germline

Project description:The male germline transcriptome changes dramatically during the mitosis-to-meiosis transition to activate late spermatogenesis genes and to transiently suppress genes commonly expressed in somatic lineages and spermatogenesis progenitor cells, termed somatic/progenitor genes. These changes reflect epigenetic regulation. Induction of late spermatogenesis genes during spermatogenesis is facilitated by poised chromatin established in the stem cell phases of spermatogonia, whereas silencing of somatic/progenitor genes during meiosis and postmeiosis is associated with formation of bivalent domains which also allows the recovery of the somatic/progenitor program after fertilization. Importantly, during spermatogenesis mechanisms of epigenetic regulation on sex chromosomes are different from autosomes: X-linked somatic/progenitor genes are suppressed by meiotic sex chromosome inactivation without deposition of H3K27me3. Our results suggest that bivalent H3K27me3 and H3K4me2/3 domains are not limited to developmental promoters (which maintain bivalent domains that are silent throughout the reproductive cycle), but also underlie reversible silencing of somatic/progenitor genes during the mitosis-to-meiosis transition in late spermatogenesis. 29 samples analyzed by ChIP-Seq

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