Project description:Gene expression analysis using nCounter immunology panel for profiling among naïve controls and immunized treatment samples. We explored the interplay between type-I IFNs and the development of host protective Th1 immunity in mice immunized with LmCen-/- parasites and challenged with L. donovani parasites and show that downregulation of IRF7 mediated production of IFN-α/β is critical for the induction of protective immunity.
Project description:microRNAs control cardiac remodeling post myocardial infarction, though the cellular and molecular mechanisms remain unclear. We used microarrays to examine microRNA profiles in mice hearts 21 days after ligation of left anterior descending coronary artery (LAD) versus sham control.
Project description:Gene expression analysis using NanoString nCounter immunology panel for profiling among naïve controls and immunized treatment samples. We explored the interplay between type-I IFNs and the development of host protective Th1 immunity in mice immunized with LmCen-/- parasites and challenged with L. donovani parasites and show that downregulation of IRF7 mediated production of IFN-α/β is critical for the induction of protective immunity.
Project description:Gene expression analysis using nCounter immunology panel for profiling among naïve controls and immunized treatment samples. We explored the interplay between type-I IFNs and the development of host protective Th1 immunity in mice immunized with LmCen-/- parasites and challenged with L. donovani parasites and show that downregulation of IRF7 mediated production of IFN-α/β is critical for the induction of protective immunity.
Project description:We report the RNA sequencing of both non-restimulated and restimulated (using anti CD3/CD28 cross-linking) antigen-experienced (CD44+) mouse lung CD8 T cells 21 days post X31 influenza A infection subsetted by integrin expression of CD49a and CD103. After restimulation, all four subsets (CD49a+CD103+, CD49a+CD103-,CD49a-CD103+, CD49a-CD103-) demonstrate global differences and separate in principle component analysis space, with CD49a+ groups showing elevated transcripts for a number of effector functions. The non-restimulated T cells separate out into CD49a+CD103+/- and CD49a-CD103+/- groups. CD49a serves as a correlate of effector transcripts in mouse lungs to a greater degree than CD103 in both non-restimulated and restimulated conditions.