Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of estrogen and testesterone on muscle primary cultures

ABSTRACT: Effect of estrogen and testesterone on muscle primary cultures

PROVIDER: PRJNA963217 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR24356000_1.fastq.gz	Fastqsanger.gz
	SRR24356000_2.fastq.gz	Fastqsanger.gz
	SRR24356001_1.fastq.gz	Fastqsanger.gz
	SRR24356001_2.fastq.gz	Fastqsanger.gz
	SRR24356002_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 60

Similar Datasets

Effect of estrogen and testesterone on muscle primary cultures

Project description:Male and female muscle primary cultures were obtained from healthy young individuals. Cultures were treated with either estrogen, testesterone or DMSO. Gene expression was measured as response to the treatment

2023-11-13 | GSE231324 | GEO

Transcriptome of mouse primary muscle cultures upon dystrophin knockdown by RNAi.

2010-12-21 | GSE20548 | GEO

Transcriptome of mouse primary muscle cultures upon dystrophin knockdown by RNAi.

Project description:Dystrophin was knocked down in primary muscle cultures prepared from C57Bl/10 neonate mice using siRNA targeting dystrophin. Also in parallel, primary muscle cultures treated with siRNA targting luciferase were used as controls. The experiment was designed so that the same cell populations were used for both test and control conditions. This helped avoid the heterogeneity associated with the previous studies where test and control cell populations were non-identical. The experiment resulted in a clear transcriptome of dystrophin deficiency and demonstrated dystrophin as a major organizer of myogensis. Moreover, several interesting experimental targets were identified which can potentially open new lines of investigations. 18 primary muscle cell cultures were prepared on 4 occasions. 7 cultures were treated with siRNAs targeting dystrophin, 7 received siRNA targeting firefly GL2 luciferase (treatment controls) and 4 remained untreated (untreated controls). RNAs were extracted 48 hours after differentiation induction for expression profiling analysis.

2010-12-21 | E-GEOD-20548 | biostudies-arrayexpress

Inflammatory response in primary muscle cell cultures in Atlantic salmon (Salmo salar)

Project description:This experiment was aimed at understanding the transcriptomic response of primary muscle cell cultures to 24h rIL-1beta stimulation

2013-12-04 | E-MTAB-1692 | biostudies-arrayexpress

The effect of estrogen on microRNA expression in primary cultured rat neonatal cardiomyocytes

Project description:The effect of estrogen on microRNA expression in primary cultured rat neonatal cardiomyocytes

| PRJNA417060 | ENA

Breast organoid suspension cultures maintain long-term estrogen receptor expression and responsiveness

Project description:Organoid cultures offer a powerful technology to investigate many different aspects of development, physiology, and pathology of diverse tissues. Unlike standard tissue culture of primary breast epithelial cells, breast organoids preserve the epithelial lineages and architecture of the normal tissue. However, existing organoid culture methods are tedious, difficult to scale, and do not robustly retain estrogen receptor (ER) expression and responsiveness in long-term culture. Here, we describe a modified culture method to generate and maintain organoids as suspension cultures. This method improves organoid growth and uniformity over the traditional Matrigel dome embedding method while still maintaining the fidelity of the three major epithelial lineages. Using this adopted method, we are able to isolate and culture hormone sensing (HS) cells alone that retain ER responsiveness upon estrogen stimulation in long-term culture. This culture system presents a valuable opportunity to study the events involved in initiation and evolution of ER-positive breast cancer.

2024-12-27 | GSE266935 | GEO

Estrogen responsive transcriptome of estrogen receptor positive normal human breast cells in 3D cultures

Project description:Estrogen responsive transcriptome of estrogen receptor positive normal human breast cells in 3D cultures

| PRJNA473820 | ENA

Estrogen-dependent gene expression in skeletal muscle

Project description:Estrogen-dependent gene expression in skeletal muscle

| PRJNA555116 | ENA

Transcriptomic profile of primary culture of skeletal muscle cells isolated from semitendinosus muscle of bulls of different performance

Project description:The goal was to examine and compare the transcriptomic profiles of primary muscle cell cultures (80% confluence) isolated from skeletal muscle (m. semitendinosus) derived from bulls belonging to different breeds and showing different levels of performance.

2020-07-29 | GSE151274 | GEO

TGFbeta effect on human skeletal muscle primary cultured cells

Project description:In this experiment we have analyzed the effect of TGFbeta-1 incubation (3h of incubation time) on the gene expression profiles of human primary cultured skeletal muscle cells, both in growing myoblasts or in differentiated muscle fibres (after 10-days of differentiation), obtained from skeletal muscle biopsies from a 2-months old healthy donor and a 5-years old DMD (Duchenne Muscular Dystrophy) patient. All human skeletal muscle biopsies were obtained by surgery from calf muscles with informed consent and approval of the Human Use Committee of Vall d'Hebron University Hospital (Barcelona, Spain). Human myoblasts were cultured on 6 cm plates until reached 70-80% of confluence and then, were incubated for 3h in the presence or in absence of TGF-?1 (2.5 ng/ml). Skeletal muscle fibres were obtained by growing human myoblasts on 6 cm plates until reached 70-80% of confluence and then, cells were induced to differentiate by deprivation of growth factors in the culture medium. After 10 days of differentiation, skeletal muscle fibers were incubated for 3h in the presence or in absence of TGFbeta-1 (2.5 ng/ml). In all cases, immediately after TGFbeta-1 treatment the RNA was extracted and used for Affymetrix microarrays analysis using the U133 2.0 microarray genechips.

2016-08-07 | E-MEXP-2937 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data