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ABSTRACT: male-mated

PROVIDER: PRJNA964572 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24557024_1.fastq.gz	Fastqsanger.gz
	SRR24557024_2.fastq.gz	Fastqsanger.gz
	SRR24557025_1.fastq.gz	Fastqsanger.gz
	SRR24557025_2.fastq.gz	Fastqsanger.gz
	SRR24557026_1.fastq.gz	Fastqsanger.gz

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Mouse utero-tubular junction (UTJ): 1: females in estrous no mating; 2: females in estrous mated with vasectomized male; 3: females in estrous mated with normal male: 1 vs. 2; 1 vs. 3; 2 vs. 3.

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2014-04-10 | GSE56620 | GEO

Paternal methamphetamine exposure differentially affects first and second generations in mice

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Mouse utero-tubular junction (UTJ): 1: females in estrous no mating; 2: females in estrous mated with vasectomized male; 3: females in estrous mated with normal male: 1 vs. 2; 1 vs. 3; 2 vs. 3.

Project description:Gene expression profiles comparing controls (1) with females mated with vasectomized male (2) or normal males (3). Three-condition experiment: 1 vs. 2; 1 vs. 3; 2 vs. 3. RNA source: 1. pool of 10 females control; 2. pool of 10 females in estrous mated with vasectomized male; 3. pool of 10 females in estrous mated with normal male.

2014-04-10 | E-GEOD-56620 | biostudies-arrayexpress

Geographical and host plant influences on transcriptional variation in Drosophila mojavensis: mapping gene expression differences in both sexes

Project description:Geographical and host plant influences on transcriptional variation in Drosophila mojavensis: mapping gene expression differences in both sexes. (1. Punta Onah:PO07; 2. Organ Pipe National Monument:OPNM08; 3. Punta Prieta:PP08; & 4. San Quintin:SQ08). The experiment was designed to investigate effects of host plant (diet), mating status (mated:M or nonmated:V) and sex (male:male or Female:F) on transcriptome.

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Project description:We present data using a novel method to simultaneously identify and quantify transferred male seminal proteins and the female reproductive proteome using multiplexed Tandem-Mass-Tag (TMT) isobaric labelling of the lower female reproductive tracts dissected from virgin- or recently mated- females of three species of the virilis group. We identified over 200 putative male ejaculate proteins many of which show differential abundance between species. We also identified over 2000 proteins providing the first description of the Drosophila female reproductive tract proteome outside of the melanogaster group which also shows significant divergence between species. We then assessed the utility of species-specific compared to single species query databases for protein identification and quantification.

2023-10-24 | PXD031638 | Pride

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2022-04-21 | PXD032157 | Pride

Roles of female and male genotype in post-mating responses in Drosophila melanogaster

Project description:Purpose: Mating induces a multitude of changes in female behavior, physiology and gene expression. Interactions between female and male genotype lead to variation in post-mating phenotypes and reproductive success. So far, few female molecules responsible for these interactions have been identified. Methods: We used Drosophila melanogaster from five geographically dispersed populations to investigate such female x male genotypic interactions at the female transcriptomic and phenotypic levels. Methods: Females from each line were singly-mated to males from the same five lines, for a total of 25 combinations. To assess whether female x male genotypic interactions affect the female post-mating transcriptome, next-generation RNA sequencing was performed on virgin and mated females at 5 to 6 hours post-mating. Results: Seventy-seven genes showed strong variation in mating-induced expression changes in a female x male genotype-dependent manner. These genes were enriched for immune response and odorant-binding functions, and for expression exclusively in the head. Conclusions: The transcriptional variation found in specific functional classes of genes might be a read-out of female x male compatibility at a molecular level. Understanding the roles these genes play in the female post-mating response will be crucial to better understand the evolution of post-mating responses and related conflicts between the sexes.

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Immunogenic males: a genome-wide analysis of reproduction and the cost of mating in Drosophila melanogaster females

Project description:In Drosophila melanogaster, mating radically transforms female physiology and behavior. Post-mating responses include an increase in the oviposition rate, a reduction in female receptivity, and an activation of the immune system . The fitness consequences of mating are similarly dramatic – females must mate once in order to produce fertile eggs, but additional matings have a clear negative effect. Previously, microarrays have been used to examine gene expression of females differing in their reproductive status with the aim of identifying genes influenced by mating. However, since only virgin and single mated females were compared, transcriptional changes associated with reproduction (under natural selection) and the effects of male-induced harm (under sexually antagonistic selection) cannot be disentangled. We partitioned these fundamentally different effects by instead examining the expression profiles of virgin, single mated and double mated females. We found substantial effects relating to reproduction and further effects that are only attributable to a second mating. Immune response genes dominate this male-induced harm effect indicating that the cost of mating may be due partly to this system's activation. We propose that both sexually antagonistic and natural selection have been important in the evolution of the innate immunity genes, thereby contributing to the sexual dimorphismand rapid evolution at these loci. Keywords: Female response to mating

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