Project description:Proper lung development and function maintenance depend on the cross-talk between resident immune cell populations and lung epithelial cells. However, understanding the underlying cellular and molecular mechanisms are difficult to study in human. Here, we present a method to develop macrophage-containing alveolar organoids from human pluripotent stem cells to investigate reciprocal interactions between alveolar macrophages and epithelial cells in lung development.
Project description:Proper lung development and function maintenance depend on the cross-talk between resident immune cell populations and lung epithelial cells. However, understanding the underlying cellular and molecular mechanisms are difficult to study in human. Here, we present a method to develop macrophage-containing alveolar organoids from human pluripotent stem cells to investigate reciprocal interactions between alveolar macrophages and epithelial cells in lung development.
Project description:scRNA-Seq of iPS cells derived multicellular human liver organoids RNA-Seq of multicellular human liver organoids derived from 3 different iPS cells
Project description:Here, we used single cell RNA-sequencing (scRNA-seq) to profile pluripotent stem cell derived human intestinal organoids (HIOs) grown in matrigel or a non-adhesive alginate hydrogel after 28 days of in vitro growth. Additionally, we used scRNA-seq to profile HIOs derived in the presence of Neuregulin 1 (NRG1) and/or EGF after 40 days of in vitro growth.
Project description:Here, we used single-cell RNA-sequencing (scRNA-seq) to profile intestinal epithelial only organoids (also known as enteroids) from human fetal duodenum after one passage of in vitro growth. Organoids were grown in the standard 25% LWRN media with either 100 ng/ml of epidermal growth factor (EGF) or 1 ng/ml of EPIREGULIN (EREG) added.
Project description:Here, we used single-cell RNA-sequencing (scRNA-seq) to profile pluripotent stem cell derived human intestinal organoids (HIOs) grown in suspension culture after 28 days of in vitro growth. Grown in minigut media supplemented with EGF.