Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA96679 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:CAF-1 is involved in nucleosome assembly following DNA replication and nucleotide excision repair. In Arabidopsis thaliana, the 3 CAF-1 subunits p150, p60 and p48 are encoded by FAS1, FAS2 and, most likely, MSI1, respectively.fas mutants exhibited increased levels of DNA double-strand breaks, a G2 phase retardation, and elevated levels of mRNAs coding for proteins involved in homologous recombinational repair. The same genes that were transcriptionally up-regulated in the fas mutants were also found to have a higher steady state level of transcription in wild-type Arabidopsis plants exposed to gamma-irradiation. Keywords: stress response, genetic modification
2006-06-20 | GSE5043 | GEO
Project description:Expression data from Arabidopsis leaves after gamma irradiation of 200 Gy
| PRJNA188202 | ENA
Project description:To analyze the genes of which their expressions are modulated by gamma irradiation, we performed the whole genome expression microarray profiling by comparing normal whole seedlings of Arabidopsis thaliana as control with those of gamma irradiated as test sample.
2019-06-30 | GSE116647 | GEO
Project description:CAF-1 is involved in nucleosome assembly following DNA replication and nucleotide excision repair. In Arabidopsis thaliana, the 3 CAF-1 subunits p150, p60 and p48 are encoded by FAS1, FAS2 and, most likely, MSI1, respectively.fas mutants exhibited increased levels of DNA double-strand breaks, a G2 phase retardation, and elevated levels of mRNAs coding for proteins involved in homologous recombinational repair. The same genes that were transcriptionally up-regulated in the fas mutants were also found to have a higher steady state level of transcription in wild-type Arabidopsis plants exposed to gamma-irradiation. Experiment Overall Design: Total RNA prepared from 4-week-old seedlings (without the roots i.e. true leaves with cotyledon and hypocotyls) using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Four-week-old sterile seedlings of wild-type plants (ecotype Nossen) were irradiated with 100 Gy of gamma-rays at a dose rate of 300 Gy/h. Six and 12 hours after irradiation, samples were frozen in liquid nitrogen and immediately ground for total RNA isolation. After confirming its quality, extracted RNA was prepared for microarray analysis. An Arabidopsis 2 Oligo Microarray kit (Agilent Technology, CA, USA) was used in this study. After confirming its quality, extracted RNA was prepared for microarray analysis as described by the manufacturer (Agilent) using the suggested chemicals and reagents.
2008-06-13 | E-GEOD-5043 | biostudies-arrayexpress
Project description:CAF-1 is a major nucleosome assembly complex, which functions particularly during replication and DNA-repair. Here, we studied how the nucleosome landscape changes in fas2 a mutant of the CAF-1 complex in the model plant Arabidopsis thaliana
2017-11-13 | GSE87421 | GEO
Project description:Mutational analysis for acute and chronic gamma irradiation in Arabidopsis
| PRJDB8649 | ENA
Project description:Upon induction of DNA damage Arabidopsis thaliana plants initiate a transcriptional response program governed by signalling cascades which are activated by the ATM and ATR kinases To analyse if the ATM dependent gene GMI1 (At5G24280) is involved in the regulation of the transcriptionl response, we compared the gene expression profiles of wildtype and gmi1 deficient plants upon gamma-irradiation
2011-04-21 | E-GEOD-23892 | biostudies-arrayexpress
Project description:Mutation detection in M1 plants after seed irradiation with gamma-rays
| PRJDB11954 | ENA
Project description:Mutational analysis of gamma-irradiated Arabidopsis seedlings
| PRJDB15059 | ENA
Project description:Arabidopsis cmt3 mutant showed neither DNA hypomethylation nor transcriptional activation of silenced repeats by gamma rays, which was found in wild type (WT). We used microarrays to compare the global transcriptional changes between the WT and cmt3 after the irradiation of 200 Gy. Transcriptions of transposition-related genes seemed to be differently affected in the WT and cmt3 by gamma rays, depending on the epigenetic codes of the genome. However, genome-wide transcriptions of the WT and cmt3 couldn't be positively correlated with the different levels of DNA methylation after the irradiation of 200 Gy. Rosette leaves from more than 10 plants were pooled for total RNA extraction and hybridization on Affymetrix Arabidopsis ATH1 genechips. We performed two independent array experiments using totally different biological replicates of WT and cmt3 mutant plants with or without gamma irradiation.
2013-06-01 | E-GEOD-43947 | biostudies-arrayexpress