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Effect of DLBCL-associated NOTCH2 mutants on genomic RBP-J localization and H3K27 acetylation

ABSTRACT: Effect of DLBCL-associated NOTCH2 mutants on genomic RBP-J localization and H3K27 acetylation

PROVIDER: PRJNA967020 | ENA |

REPOSITORIES: ENA

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Effect of DLBCL-associated NOTCH2 mutants on genomic RBP-J localization and H3K27 acetylation

Project description:Assessment of the genome-wide effect of DLBCL-asscociated NOTCH2 mutants in DLBCL on RBPJ and H3K27acetylation. DLBCL cell lines U2932 cells stably expressing HA-tagged NOTCH2(WT), NOTCH2(R2400*) or NOTCH2(Q2140*) were subjected to chromatin immnunoprecipitation DNA-sequencing (ChIP-seq) for RBP-J and H3K27ac.

2024-05-03 | GSE231623 | GEO

(1) Effect of KLHL6 loss on the transcriptome in DLBCL upon NOTCH ligand stimulation. (2) Effect of KLHL6 and NOTCH2 loss on the transcriptome in DLBCL. (3) Effect of DLBCL-associated NOTCH2 mutants on the transcriptome in DLBCL.

Project description:(1) Assessment of the transcriptional changes regulated by KLHL6 upon NOTCH ligand stimulation. Bulk RNA-seq in KLHL6 WT or KO U2932 cells was performed upon incubation with stromal OP9 cells stably expressing NOTCH ligand DLL1. (2) Assessment of the transcriptional changes regulated by the KLHL6-NOTCH2 axis in a xenotransplant model of DLBCL. Bulk RNA-seq was performed in in KLHL6+/+NOTCH2+/+(WT), KLHL6-/-NOTCH2+/+(K6-KO), KLHL6+/+NOTCH2-/-(N2-KO), KLHL6-/-NOTCH2-/-(DKO) U2932 cells xenotranplanted in NSG mice trated with CHOP. (3) Assessment of the transcriptional changes regulated by the DLBCL-associated NOTCH2 mutants. Bulk RNA-seq was performed inU2932 cells stably expressing NOTCH2(WT), NOTCH2(S2156A), NOTCH2(R2400*) or NOTCH2(Q2140*).

2024-05-03 | GSE231624 | GEO

Project description:(1) Effect of KLHL6 loss on the transcriptome in DLBCL upon NOTCH ligand stimulation. (2) Effect of KLHL6 and NOTCH2 loss on the transcriptome in DLBCL. (3) Effect of DLBCL-associated NOTCH2 mutants on the transcriptome in DLBCL.

| PRJNA967024 | ENA

NOTCH2 mutants

Project description:NOTCH2 mutants

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Transcriptome changes caused by RBP-L knockdown in rice grains

Project description:A T-DNA insertion within RBP-L 3’UTR resulted in 10-25% expression level of RBP-L gene compared to wild-type. The reduced expression of RBP-L caused partial mis-localization of glutelin and prolamine RNAs and conferred other general growth defects including dwarfism, late flowering and smaller seeds. Transcriptome analysis showed that RBP-L knockdown greatly affected the expression of prolamine family genes and many genes invovled in essential biological pathways during plant development.

2019-05-22 | GSE124301 | GEO

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Project description:DLBCL

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Effect of Notch2 in chondrocytes

Project description:To investigate the role of Notch2 in the inflammatory response in chondrocytes, we cultured primary chondrocytes derived from wild type and Notch2 mutant mice and treated TNFalpha or vehicle. We then performed gene expression profiling analysis using data obtained from RNA-seq of wild type and Notch2 mutant chondrocytes.

2023-10-16 | GSE224255 | GEO

Notch2 controls non-autonomous Wnt-signalling in chronic lymphocytic leukaemia

Project description:The Wnt-signalling pathway is one of the core de-regulated pathways in chronic lymphocytic leukemia (CLL), activated in a subset of patients by somatic coding mutations. Here we describe an alternative mechanism of Wnt-activation in malignant B cells, mediated by Notch2 activity in mesenchymal stromal cells (MSC) in the tumor microenvironment. We identified that tumor cells specifically induce and activate Notch2 in MSCs. Notch2 orchestrates the expression of target genes essential for the activation of canonical Wnt-signaling in CLL cells. Mechanistically, stromal Notch2 mediates the stabilization of â-catenin by inhibiting the activation of Gsk3-â in malignant B cells. Pharmacological inhibition of the Wnt-pathway mitigates microenvironment-mediated survival of malignant B cells in vitro. Similarly, inhibition of Notch-signaling impaired survival of CLL cells and disease engraftment in a PDX mouse model. Notch2 activation in the tumour microenvironment is a pre-requisite for the GSK3-â dependent activation of the canonical Wnt-signaling in tumor cells.

2018-09-19 | PXD010401 | Pride

DLBCL patients

Project description:DLBCL patients

| PRJNA613053 | ENA

Transcriptional impact of conditional loss of Notch2 from adult NSCs

Project description:Adult NSCs maintenance depends on intakt Notch signaling. The role of Notch2 has only been marginally studied in adult neurogenesis. To adress the role of the Notch2 paralogue we conditionally deleted Notch2 from the adult SVZ and analysed the Notch2-deficient cells at distinct time points.

2018-01-23 | GSE99916 | GEO

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