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NRF1 promotes primordial germ cell development, proliferation, and survival [RNA-seq]

ABSTRACT: NRF1 promotes primordial germ cell development, proliferation, and survival [RNA-seq]

PROVIDER: PRJNA967271 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24433036_1.fastq.gz	Fastqsanger.gz
	SRR24433036_2.fastq.gz	Fastqsanger.gz
	SRR24433037_1.fastq.gz	Fastqsanger.gz
	SRR24433037_2.fastq.gz	Fastqsanger.gz
	SRR24433038_1.fastq.gz	Fastqsanger.gz

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NRF1 promotes primordial germ cell development, proliferation, and survival

Project description:NRF1 promotes primordial germ cell development, proliferation, and survival

| PRJNA967266 | ENA

NRF1 promotes primordial germ cell development, proliferation, and survival [RNA-seq]

Project description:To investigate the role of NRF1 in regulating primordial germ cell development, We established conditional knockout mice of Nrf1 in primordial germ cell to observe the effect of Nrf1 knockout on the development of primordial germ cell. At the same time, we utilized a pluripotent stem cell differentiation system in vitro to obtain PGCL cells for chip_ Seq, analyze which genes Nrf1 directly binds to. Meanwhile, we established a pluripotent stem cell line induced by Nrf1 overexpression and performed RNA_seq analysis on PGCL cells overexpressing Nrf1 obtained in vitro

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NRF1 promotes primordial germ cell development, proliferation and survival [ChIP-seq]

2023-08-05 | GSE231661 | GEO

NRF1 promotes primordial germ cell development, proliferation and survival [ChIP-seq]

Project description:NRF1 promotes primordial germ cell development, proliferation and survival [ChIP-seq]

| PRJNA967267 | ENA

NRF1 promotes primordial germ cell development, proliferation, and survival

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-08-05 | GSE231663 | GEO

Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro.

Project description:Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro.

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MCM8 interacts with DDX5 to promote R-loop resolution in primordial germ cells

Project description:MCM8 has emerged as a core gene in reproductive aging and is crucial for meiotic homologous recombination repair. It also safeguards genome stability by coordinating the replication stress response during mitosis, but its function in mitotic germ cells remains elusive. Here we found that the number of primordial germ cells (PGCs) decreased dramatically after MCM8 deficiency due to proliferation defects. We further demonstrated that MCM8 interacted with two known helicases DDX5 and DHX9 in PGCs, and loss of MCM8 led to R-loop accumulation by reducing the retention of these helicases at R-loops, thus inducing genome instability. Cells expressing premature ovarian insufficiency-causative mutants of MCM8 with decreased interaction with DDX5 displayed increased R-loop levels. These results suggest that MCM8 interacts with R-loop-resolving factors to prevent DNA damage in PGCs, thus maintaining PGC proliferation and establishing the reproductive reserve. Our findings thus reveal a novel role for MCM8 in PGC development and improve our understanding of reproductive aging caused by genome instability in mitotic germ cells.

2024-05-31 | PXD050727 | Pride

Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro [bis-seq]

Project description:Primordial germ cells (PGCs) are the founding population of the germ cell lineage that undergo a multistep process to generate spermatozoa or oocytes. Establishing an appropriate culture system for PGCs is a key challenge in reproductive biology. By a chemical screening using mouse PGC-like cells (mPGCLCs), which were induced from mouse embryonic stem cells, we reported previously that forskolin and rolipram synergistically enhanced the proliferation/survival of mPGCLCs with an average expansion rate of ∼20-fold. In the present study, we evaluated other chemicals or cytokines to see whether they would improve the current mPGCLC culture system. Among the chemicals and cytokines examined, in the presence of forskolin and rolipram, cyclosporin A (CsA) and fibroblast growth factors (FGFs: FGF2 and FGF10) effectively enhanced the expansion of mPGCLCs in vitro (∼50-fold on average). During the expansion by CsA or FGFs, mPGCLCs comprehensively erased their DNA methylation to acquire a profile equivalent to that of gonadal germ cells in vivo, while maintaining their highly motile phenotype as well as their transcriptional properties as sexually uncommitted PGCs. Importantly, these mPGCLCs robustly contributed to spermatogenesis and produced fertile offspring. Furthermore, mouse PGCs (mPGCs) cultured with CsA ex vivo showed transcriptomes and DNA methylomes similar to those of cultured mPGCLCs. The improved culture system for mPGCLCs/mPGCs would be instructive for addressing key questions in PGC biology, including the mechanisms for germ cell migration, epigenetic reprogramming, and sex determination of the germline.

2021-02-16 | GSE151445 | GEO

Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro [RNA-seq]

2021-02-16 | GSE151444 | GEO

Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro [RNA-seq]

Project description:Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro [RNA-seq]

| PRJNA635877 | ENA

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