Project description:H3K27ac Hi-ChIP analysis was performed in Jurkat and RPMI-8402 stably expressing dCAR9-KRAB after treatment with DMSO (Control) or Doxycycline to induce sgRNA expression to block MYCN enhnacer region
Project description:Cut&Run analysis was performed in RPMI-8402 cells to analyze binding regions of MYC, MYCN and various histone modifications in normal culture conditions
Project description:The oncogenic transcription factor TAL1/SCL is aberrantly overexpressed in over 40% of cases of T-cell acute lymphoblastic leukemia (T-ALL), emphasizing the importance of the TAL1-regulated transcriptional program in the molecular pathogenesis of T-ALL. Here we identify regions occupied by TAL1 and its regulatory partners HEB, E2A, and GATA3 in a T-ALL cell line (RPMI-8402).