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SCR106 splicing factor modulates abiotic stress responses by maintaining the RNA splicing in rice

ABSTRACT: SCR106 splicing factor modulates abiotic stress responses by maintaining the RNA splicing in rice

PROVIDER: PRJNA971147 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24492330_1.fastq.gz	Fastqsanger.gz
	SRR24492330_2.fastq.gz	Fastqsanger.gz
	SRR24492331_1.fastq.gz	Fastqsanger.gz
	SRR24492331_2.fastq.gz	Fastqsanger.gz
	SRR24492332_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

SCR106 splicing factor modulates abiotic stress responses by maintaining the RNA splicing in rice

Project description:We investigated the function of the plant-specific SR protein SC106 in pre-mRNA splicing regulation and abiotic stress responses in rice. The loss-of-function mutant, SC106, showed increased sensitivity to salt. We performed genome-wide analyses of gene expression and splicing in seedlings subjected to salt stress and identified multiple splice isoforms from stress-responsive genes dependent on SC106.

2023-10-26 | GSE232160 | GEO

SCR106 splicing factor modulates abiotic stress responses by maintaining RNA splicing in rice.

Project description:Plants employ sophisticated molecular machinery to fine-tune their responses to growth, developmental, and stress cues. Gene expression influences plant cellular responses through regulatory processes such as transcription and splicing. Pre-mRNA is alternatively spliced to increase the genome coding potential and further regulate expression. Serine/arginine-rich (SR) proteins, a family of pre-mRNA splicing factors, recognize splicing cis-elements and regulate both constitutive and alternative splicing. Several studies have reported SR protein genes in the rice genome, subdivided into six subfamilies based on their domain structures. Here, we identified a new splicing factor in rice with an RNA recognition motif (RRM) and SR-dipeptides, which is related to the SR proteins, subfamily SC. OsSCR106 regulates pre-mRNA splicing under abiotic stress conditions. It localizes to the nuclear speckles, a major site for pre-mRNA splicing in the cell. The loss-of-function scr106 mutant is hypersensitive to salt, abscisic acid, and low-temperature stress, and harbors a developmental abnormality indicated by the shorter length of the shoot and root. The hypersensitivity to stress phenotype was rescued by complementation using OsSCR106 fused behind its endogenous promoter. Global gene expression and genome-wide splicing analysis in wild-type and scr106 seedlings revealed that OsSCR106 regulates its targets, presumably through regulating the alternative 3'-splice site. Under salt stress conditions, we identified multiple splice isoforms regulated by OsSCR106. Collectively, our results suggest that OsSCR106 is an important splicing factor that plays a crucial role in accurate pre-mRNA splicing and regulates abiotic stress responses in plants.

| S-EPMC10837019 | biostudies-literature

A plant-specific splicing factor RS33 regulates abiotic stress responses in rice

Project description:Here, we investigated the function of the plant-specific SR protein RS33 in pre-mRNA splicing regulation and abiotic stress responses in rice. The loss-of-function mutant, rs33, showed increased sensitivity to salt and low-temperature stress. Genome-wide analyses of gene expression and splicing in seedlings subjected to these stresses identified multiple splice isoforms from stress-responsive genes dependent on RS33. The number of RS33-regulated genes is much higher under low-temperature stress as compared to salt stress. Our results suggest that this plant-specific splicing factor plays crucial and distinct roles during plant adaptation to abiotic stresses.

2022-06-15 | GSE194283 | GEO

A plant-specific splicing factor RS33 regulates abiotic stress responses in rice

Project description:A plant-specific splicing factor RS33 regulates abiotic stress responses in rice

| PRJNA800054 | ENA

The Rice Serine/Arginine Splicing Factor RS33 Regulates Pre-mRNA Splicing during Abiotic Stress Responses.

Project description:Abiotic stresses profoundly affect plant growth and development and limit crop productivity. Pre-mRNA splicing is a major form of gene regulation that helps plants cope with various stresses. Serine/arginine (SR)-rich splicing factors play a key role in pre-mRNA splicing to regulate different biological processes under stress conditions. Alternative splicing (AS) of SR transcripts and other transcripts of stress-responsive genes generates multiple splice isoforms that contribute to protein diversity, modulate gene expression, and affect plant stress tolerance. Here, we investigated the function of the plant-specific SR protein RS33 in regulating pre-mRNA splicing and abiotic stress responses in rice. The loss-of-function mutant rs33 showed increased sensitivity to salt and low-temperature stresses. Genome-wide analyses of gene expression and splicing in wild-type and rs33 seedlings subjected to these stresses identified multiple splice isoforms of stress-responsive genes whose AS are regulated by RS33. The number of RS33-regulated genes was much higher under low-temperature stress than under salt stress. Our results suggest that the plant-specific splicing factor RS33 plays a crucial role during plant responses to abiotic stresses.

| S-EPMC9180459 | biostudies-literature

AtBRO1, BRO1-like domain-containing protein, modulates growth and abiotic stress responses in Arabidopsis

Project description:AtBRO1, BRO1-like domain-containing protein, modulates growth and abiotic stress responses in Arabidopsis

| PRJNA933625 | ENA

PRMT5 Modulates Splicing in Hematopoietic Stem Cells

Project description:PRMT5 Modulates Splicing in Hematopoietic Stem Cells

| PRJNA479853 | ENA

Distinct proteomic responses to multiple abiotic stresses in two rice genotypes

Project description:In the present study, we found that Nipponbare and IAC1131 behave equivalently in response to multiple abiotic stress, which included simultaneous application of drought, salt and temperature stress. At the level of molecular analysis by isotopic labelling quantitative proteomics, 6215 proteins were reproducibly identified and quantified across the two genotypes and three time points sampled. Heat shock proteins, late embryogenesis abundant proteins, and photosynthesis-related proteins were all found to be differentially abundant in response to stress treatments in both Nipponbare and IAC1131.

2022-02-22 | PXD030428 | Pride

AtBRO1, BRO1-like domain-containing protein, modulates growth and abiotic stress responses in Arabidopsis

Project description:Plants physiological mechanisms are affected by Abscisic acid (ABA) via changing gene expression and empowers plants to adapt in numerous environments. Plants have evolved their protection mechanisms for seed germination and abiotic environments to deal with critical harsh conditions. Here, we have explored those changes in Arabidopsis thaliana plants subjected to multiple abiotic stresses. AtBro1 transcripts showed up-regulation in the presence of salt, ABA and mannitol stress conditions. AtBRO1 over-expression lines exhibited robust tolerance to drought and salt stress. Further, ABA elicits resistance responses in loss-of-function bro1-1 mutant plants and AtBro1 positively regulates drought resistance in Arabidopsis. Promoter of AtBro1 fused with GUS showed GUS expression mainly in the rosette leaves and floral clusters, especially in anthers. AtBro1 protein was found to be localized in the plasma membrane of Arabidopsis at the subcellular level by using AtBro1::GFP fusion. Using a broad RNA-sequencing analysis, we observed that early transcriptional responses prompted by ABA induction exhibit specific quantitative differences at different time points, suggesting that ABA stimulates resistance responses in bro1-1 mutant plants. Additionally, transcripts levels of MOP9.5, MRD1, HEI10, and MIOX4 were altered in loss-of-function mutant plants which are involved in different stress conditions. Collectively, our results have shown that AtBro1 is involved in a significant role by regulating plant transcriptional response to ABA and induction of resistance response against abiotic stress.

2023-04-18 | GSE225062 | GEO

An SWAP1-SFPS-RRC1 splicing factor complex modulates pre-mRNA splicing to promote photomorphogenesis in Arabidopsis

Project description:To investigate the molecular and physiological functions of SWAP1 in light signaling. We show that SWAP1 modulates global gene expression and alternative splicing both under dark and red light treated conditions

2022-11-09 | GSE214299 | GEO

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