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Effects of mTOR inhibition on the phenotypes of spleen naive CD4+ T (TN) cells.

ABSTRACT: Effects of mTOR inhibition on the phenotypes of spleen naive CD4+ T (TN) cells.

PROVIDER: PRJNA971900 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24520450_1.fastq.gz	Fastqsanger.gz
	SRR24520450_2.fastq.gz	Fastqsanger.gz
	SRR24520451_1.fastq.gz	Fastqsanger.gz
	SRR24520451_2.fastq.gz	Fastqsanger.gz
	SRR24520452_1.fastq.gz	Fastqsanger.gz

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Effects of mTOR inhibition on the phenotypes of spleen naive CD4+ T (TN) cells.

Project description:To analyze the influence of mTOR activity on TN cells, RNA-seq analysis was performed on splenic TN cells from mice treated or not treated with the mTOR inhibitor Rapamycin.

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Comparison of mRNA expression between spleen and mesenteric lymph node (mLN) naive CD4+ T (TN) cells

Project description:To analyze difference between spleen and mLN TN cells, RNA-seq analysis was performed on TN cells freshly prepared from each organ.

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RNA-seq analysis of spleen and mesenteric lymph node (mLN) hematopoietic cells

Project description:To search for factors that affect naive CD4+ T (TN) cell phenotypes in spleen, we performed RNA-seq analysis to compare mRNA expression between freshly-prepared spleen and mLN hematopoietic cells.

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Elucidation of the effects of tonic TCR stimulation and tissue environments of the chromatin status of naive CD4 T (TN) cells

Project description:Naive CD4 T (TN) cells show some degree of heterogeneity. However, mechanisms that alter TN cells are still largely unknown, and physiological importance in the phenotypic alteration of TN cells is unclear. Thus, to reveal the mechanisms that alter TN cell characteristics, we performed transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) on TN cells from different secondary lymphoid organs with different strength of tonic TCR stimulation.

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Human Naive Pluripotency Attained by Transient Inhibition of mTOR

Project description:Mouse embryonic stem cells (mESCs) are in naive pluripotency that represents the ground state of development, from which all cells in the mouse embryo are derived. In contrast, human embryonic stem cells (hESCs) are in a primed state of pluripotency with many different properties. Despite intense efforts to generate naive human pluripotent stem cells (hPSCs), it has not been possible to derive naive hPSCs without relying on transgene overexpression or chemicals. Here, we show that a transient treatment with Torin1, a selective inhibitor of mTOR, converted hPSCs from primed to naive pluripotency. The naive hPSCs were maintained in the same condition as mESCs in defined media with 2iLI (MEK inhibitor, GSK3b inhibitor, LIF and Insulin). Like mESCs, they exhibited high clonal efficiency, rapid cell proliferation, active mitochondrial respiration, X chromosome activation, DNA hypomethylation, and transcriptomes similar to those of human blastocysts than primed hESCs. Most importantly, the naive hPSCs significantly contributed to mouse embryos when transferred to mouse blastocysts. mTor inhibition induced nuclear translocation of TFE3, a critical transcription factor at the interplay of autophagy and pluripotency. TFE3 with mutated nuclear localization signal blocked the conversion from primed to naive pluripotency. It appears that by mimicking diapause at the cellular level, naive pluripotency in human can be readily attained from primed hPSCs, thus establishing the unified ground state of pluripotency in mammals.

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Human Naive Pluripotency Attained by Transient Inhibition of mTor

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Human Naive Pluripotency Attained by Transient Inhibition of Mtor

Project description:Human Naive Pluripotency Attained by Transient Inhibition of Mtor

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