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Comparative single-cell analyses identify shared and divergent features of human and mouse kidney development [mouse snATAC-seq]

ABSTRACT: Comparative single-cell analyses identify shared and divergent features of human and mouse kidney development [mouse snATAC-seq]

PROVIDER: PRJNA972294 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24542230_1.fastq.gz	Fastqsanger.gz
	SRR24542230_2.fastq.gz	Fastqsanger.gz
	SRR24542231_1.fastq.gz	Fastqsanger.gz
	SRR24542231_2.fastq.gz	Fastqsanger.gz
	SRR24542232_1.fastq.gz	Fastqsanger.gz

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Comparative single-cell analyses identify shared and divergent features of human and mouse kidney development [mouse snATAC-seq]

Project description:Mammalian kidneys maintain fluid homeostasis through the cellular activity of nephrons and the conjoined collecting system. Each epithelial network originates from distinct progenitor cell populations that reciprocally interact during development. To extend our understanding of human and mouse kidney development, we profiled chromatin organization (ATAC-seq) and gene expression (RNA-seq) in developing human and mouse kidneys. Data were analyzed at a species level and then integrated into a common, cross-species multimodal data set. Comparative analysis of cell types and developmental trajectories identified conserved and divergent features of chromatin organization and linked gene activity, revealing species- and cell-type specific regulatory programs. Identification of human-specific enhancer regions linked through GWAS studies to kidney disease highlights the potential of developmental modeling to provide clinical insight.

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Comparative single-cell analyses identify shared and divergent features of human and mouse kidney development

Project description:Comparative single-cell analyses identify shared and divergent features of human and mouse kidney development

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Comparative single-cell analyses identify shared and divergent features of human and mouse kidney development [mouse scRNA-seq]

Project description:Comparative single-cell analyses identify shared and divergent features of human and mouse kidney development [mouse scRNA-seq]

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Comparative single-cell analyses identify shared and divergent features of human and mouse kidney development [human snRNA-seq]

Project description:Comparative single-cell analyses identify shared and divergent features of human and mouse kidney development [human snRNA-seq]

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Comparative single-cell analyses identify shared and divergent features of human and mouse kidney development [human ATAC-seq]

Project description:Comparative single-cell analyses identify shared and divergent features of human and mouse kidney development [human ATAC-seq]

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Comparative single-cell analyses identify shared and divergent features of human and mouse kidney development [human snRNA-seq]

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snATAC-seq of the developing mouse cerebellum across eleven stages

Project description:This study used droplet-based snATAC-seq to profile the chromatin accessibility landscape of 91,922 nuclei in the mouse cerebellum across eleven developmental stages, from the beginning of neurogenesis (e10.5) till adulthood (P63). The study included two biological replicates per stage, one from each sex. Cerebelli were dissected as whole or in two halves, nuclei were extracted and profiled using 10x single-cell ATAC reagent kit (v1.0) and a Chromium controller. Libraries were sequenced using paired-reads on Illumina NextSeq 550 and initial data processing was performed using Cellranger ATAC (1.1).

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Comparative single-cell analyses identify shared and divergent features of human and mouse kidney development

Project description:This SuperSeries is composed of the SubSeries listed below.

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