Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of Tet2 deletion on gene expression in mice

ABSTRACT: Effect of Tet2 deletion on gene expression in mice

PROVIDER: PRJNA974430 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Effect of Tet2 deletion on gene expression in mice

Project description:To investigate the effect of Tet2 on gene expression, we established Nestin Cre+ Tet2lox/lox (Tet2 cKO) mice and Nestin Cre- Tet2lox/lox (WT) mice , collected their habenular tissue, and compared the differential gene expressions at mRNA levels. We then performed gene expression profiling analysis using data obtained from RNA-seq in the habenular of 2-month mice.

2023-05-24 | GSE232920 | GEO

Effect of hematopoietic Tet2 deletion on steatohepatitis

Project description:Effect of hematopoietic Tet2 deletion on steatohepatitis

| PRJNA927295 | ENA

Effect of Tet2 deletion on gene expression in liver macrophages in a mouse model of steatohepatitis [sorted]

Project description:To investigate the role of hematopoietic Tet2 loss on the development of steatohepatitis, mice were transplanted with wild type or Tet2 deficient bone marrow cells and fed a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD). Gene expression profiling analysis of sorted liver macrophages was performed.

2023-02-07 | GSE223694 | GEO

Effect of hematopoietic Tet2 deletion on bulk liver gene expression in a mouse model of steatohepatitis [bulk]

Project description:To investigate the role of hematopoietic Tet2 loss on the development of steatohepatitis, mice were transplanted with wild type or Tet2 deficient bone marrow cells and fed a choline-deficient, L-amino acid-defined, high-fat diet. Gene expression profiling analysis of bulk liver RNA was then performed.

2023-02-07 | GSE223692 | GEO

Tet2-mediated DNA demethylation controls T cell cytokine expression [array]

Project description:The conversion of 5-methylcytosine (5mC) into 5-Hydroxymethylcytosine (5hmC) by ten-eleven translocation (Tet) family has recently been identified as a key process for active DNA demethylation, whose effects in the immune response is currently unknown. We used microarrays to characterize the regulation of Tet2 in T cells. We found that deletion of the Tet2 gene in T cells decreased expression of effector cytokines such as IFN-?, IL-17, and IL-10. To analyze the regulation of Tet2 in Th subset differentation, CD2(Cre)Tet2(f/f) mice were used to derive Tet2-deficient Th1 and Th17 cells, and Tet2(f/f) mice were used for Tet2-enriched Th1 and Th17 cells.

2015-04-12 | E-GEOD-48657 | biostudies-arrayexpress

Effect of hematopoietic Tet2 deletion on bulk liver gene expression in a mouse model of steatohepatitis [bulk]

Project description:Effect of hematopoietic Tet2 deletion on bulk liver gene expression in a mouse model of steatohepatitis [bulk]

| PRJNA927300 | ENA

Effect of Tet2 deletion on gene expression in liver macrophages in a mouse model of steatohepatitis [sorted]

Project description:Effect of Tet2 deletion on gene expression in liver macrophages in a mouse model of steatohepatitis [sorted]

| PRJNA927302 | ENA

Effect of Notch4 deletion on pancreatic tumorigenesis in mice

Project description:Effect of Notch4 deletion on pancreatic tumorigenesis in mice

| PRJNA762919 | ENA

effect of slc11a2 deletion on placental gene expression

Project description:effect of slc11a2 deletion on placental gene expression

| PRJNA973089 | ENA

Combined loss of Tet1 and Tet2 promotes B-cell, but not myeloid malignancies in mice

Project description:TET1/2/3 are methylcytosine dioxygenases regulating cytosine hydroxymethylation in the genome. Tet1 and Tet2 are abundantly expressed in HSC/HPCs and implicated in the pathogenesis of hematological malignancies. Tet2-deletion in mice causes myeloid malignancies, while Tet1-null mice develop B-cell lymphoma after an extended period of latency. Interestingly, TET1 and TET2 were often concomitantly down-regulated in acute B-lymphocytic leukemia. Here, we investigated the overlapping and non-redundant functions of Tet1/Tet2 in HSC maintenance and development of hematological malignancies using Tet1/2 double knockout (DKO) mice. DKO and Tet2-/- HSC/HPCs had overlapping and unique 5hmC and 5mC profiles and behaved differently. DKO mice exhibited strikingly decreased incidence and delayed onset of myeloid malignancies compared to Tet2-/- mice and in contrast developed lethal B-cell malignancies. Transcriptome analysis of DKO tumors revealed expression changes in many genes dysregulated in human B-cell malignancies, such as LMO2, BCL6 and MYC. These results highlight the critical roles of TET1 or TET2 individually and their cross-talks in the pathogenesis of hematological malignancies. Given the role of Tet proteins in 5mC oxidation, we employed a previously established chemical labeling and affinity purification method coupled with high-throughput sequencing (hMe-Seal) to profile the genome-wide distribution of 5hmC, as well as methylated DNA immunoprecipitation (MeDIP) coupled with high-throughput sequencing (MeDIP-seq) to profile 5mC using BM LK cells purified from young WT, Tet2-/- and DKO mice (6-10 wks old).

2015-11-12 | E-GEOD-73611 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data