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Effect of WNT hyperactivation by CTNNB1 overexpression or APC knockdown on gene expression in HT29 cells

ABSTRACT: Effect of WNT hyperactivation by CTNNB1 overexpression or APC knockdown on gene expression in HT29 cells

PROVIDER: PRJNA974459 | ENA |

REPOSITORIES: ENA

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Effect of WNT hyperactivation by CTNNB1 overexpression or APC knockdown on gene expression in HT29 cells

Project description:To investigate effect of WNT hyperactivation by CTNNB1 overexpression or APC knockdown on gene expression and probe possible mechanism of cell viability effect, we overexpressed GFP/CTNNB1, or induce knockdown of APC by shNT1 (non-targeting) and shAPC

2023-07-17 | GSE232944 | GEO

Changes in the transcriptome of primary mouse mammary gland organoids in response to hyperactivation of the WNT/CTNNB1 pathway

Project description:Purpose: WNT/CTNNB1 signaling promotes mammary tumor formation in mice, but the direct and immediate effects of elevated levels of WNT/CTNNB1 signaling on the mouse mammary epithelium remain unknown. Using primary mouse mammary epithelial organoid cultures grown in minimal media we have studied the response of the mouse mammary epithelium to elevated levels of WNT/CTNNB1 signaling.

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USP7 is a tumor-specific WNT activator for APC-mutated colorectal cancer by mediating B-catenin deu

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Project description:To elucidate the role(s) of WNT/CTNNB1 signaling in the testis, transgenic Ctnnb1 tm1Mmt/+;Amhr2 tm3(cre)Bhr/+ mice were generated to obtain sustained activation of the WNT/CTNNB1 pathway in Sertoli cells. Male Ctnnb1 tm1Mmt/+;Amhr2 tm3(cre)Bhr/+ mice were sterile because of testicular atrophy starting at 5 wk of age, associated with degeneration of seminiferous tubules and the progressive loss of germ cells. Sustained WNT/CTNNB1 pathway activation was obtained in Ctnnb1 tm1Mmt/+;Amhr2 tm3(cre)Bhr/+ Sertoli cells. Sertoli cells often exhibited morphological characteristics suggestive of incomplete differentiation that appeared in a manner coincident with germ cell loss, accompanied by an increase in the expression of the immature Sertoli cell marker AMH. Microarray analyses performed on cultured Sertoli cells showed that CTNNB1 induces the expression of genes associated with the female sex determination pathway as well as cell-cycle associated genes . Together, these data suggest that the WNT/CTNNB1 pathway regulates Sertoli cell functions critical to their capacity to support spermatogenesis and to proliferate in the postnatal testis. Sertoli cells were obtained from 3wks-old Ctnnb1(tm1Mmt/tm1Mmt) mice. Cells were infected with adenoviruses to express eGFP or Cre in serum-free medium, and subsequently harvested for RNA extraction. Preliminary experiments demonstrated that an infection efficiency of nearly 100% could be obtained at an MOI of 50 (as determined by analysis of fluorescent signal in Ad-eGFP-infected cells) and that the Ad-Cre virus produced complete recombination of the floxed Ctnnb1 allele after 12 hours. Microaaray analyses were done using triplicate RNA samples from each adenoviral treatment using MouseRef-6 v.2.0 expression BeadChips technology (Illumina, San Diego, CA).

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Seminiferous tubule degeneration and infertility in mice with sustained activation of WNT/CTNNB1 signaling in sertoli cells

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Changes in the transcriptome of primary mouse mammary gland organoids in response to hyperactivation of the WNT/CTNNB1 pathway

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Transcription profiling by array of Apc-deficient mouse small intestinal epithelium upon Msx1 depletion.

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2019-11-01 | E-MTAB-6930 | biostudies-arrayexpress

Transcription profiling by array of Apc-deficient proximal colonic epithelium upon Msx1 transcription factor depletion

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