Project description:Transcriptional profiling of Lactobacillus delbrueckii subsp. bulgaricus 2038 during the growth in casein proteins conditioned medium compared with the start control (cells treated in whey conditioned medium). Identifying the genes that are differentially expressed during the growth of Lb. bulgaricus 2038 in casein proteins condition provides a starting point for the investigation of metabolic mechanisms.
Project description:Transcriptional profiling of Lactobacillus delbrueckii subsp. bulgaricus 2038 during the growth in casein proteins conditioned medium compared with the start control (cells treated in whey conditioned medium). Identifying the genes that are differentially expressed during the growth of Lb. bulgaricus 2038 in casein proteins condition provides a starting point for the investigation of metabolic mechanisms. Twelve Samples at different growth time points. Three replicates each.
Project description:The objective of this study was to decipher the metabolism expressed by Lactobacillus delbrueckii subsp. delbrueckii CIRM-BIA865 during soy juice fermentation using transcriptomics. The whole genome was sequenced, assembled and annotated. CIRM-BIA865 was then used to ferment soy juice to produce a soy-based yogurt. Samples were analysed in kinetics during fermentation, at pH values of 6.5, 6, 5 and 4.6. RNA from CIRM-BIA865 were extracted and sequenced using paired-end Illumina. Reads were mapped using Bowtie2 on previously obtained genome of CIRM-BIA865. No mismatch were allowed. Reads mapped on CDS were counted using htseqcount.List of differentially expressed (DE) genes between two successive sampling times (determined by pH) were generated using DEseq2 with a modified t-test and a p-value adjusted by Bonferoni inferior to 0.05. Fold changes expressed how many times genes were induced along the fermentations.
Project description:We performed the long-term administration experiment using a yogurt fermented with Lactobacillus delbrueckii subsp. bulgaricus 2038 and Streptococcus thermophilus 1131 (LB81 yogurt) for 20 months in order to understand the effects of the long-term intake of probiotics on mice. Microarrays were used to compare the gene expressions of the intestine, liver and spleen tissues between control mice and LB81 yogurt-intake mice at 28 months of age. Abbreviations used: A, LB81 yogurt-intake mice; C, control mice