Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of cardiac developmental toxicants in differentiated human induced pluripotent stem cells [Toxicants]

ABSTRACT: Identification of cardiac developmental toxicants in differentiated human induced pluripotent stem cells [Toxicants]

PROVIDER: PRJNA978589 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Identification of cardiac developmental toxicants in differentiated human induced pluripotent stem cells

Project description:Identification of cardiac developmental toxicants in differentiated human induced pluripotent stem cells

| PRJNA978583 | ENA

Identification of cardiac developmental toxicants in differentiated human induced pluripotent stem cells [CHIR]

Project description:Identification of cardiac developmental toxicants in differentiated human induced pluripotent stem cells [CHIR]

| PRJNA978588 | ENA

Identification of cardiac developmental toxicants in differentiated human induced pluripotent stem cells [Toxicants]

Project description:Animal studies for embryotoxicity evaluation of potential therapeutics and environmental factors are complex, costly, and time-consuming. Often, studies are not of human relevance because of species differences. In the present study, we recapitulated the process of cardiomyogenesis in human induced pluripotent stem cells (hiPSCs) by modulation of the Wnt signaling pathway to identify a key cardiomyogenesis gene signature that can be applied to identify compounds and/or stress factors compromising the cardiomyogenesis process. Among the 23 tested teratogens and 16 non-teratogens, we identified three retinoids including 13-cis-retinoic acid that completely block the process of cardiomyogenesis in hiPSCs. Moreover, we have identified an early gene signature consisting of 31 genes and associated biological processes that are severely affected by the retinoids. To predict the inhibitory potential of teratogens and non-teratogens in the process of cardiomyogenesis we established the “Developmental Cardiotoxicity Index” (DCI_31g) that accurately differentiates teratogens and non-teratogens to do or do not affect the differentiation of hiPSCs to functional cardiomyocytes.

2023-06-06 | GSE233924 | GEO

Identification of cardiac developmental toxicants in differentiated human induced pluripotent stem cells [CHIR]

2023-06-06 | GSE233925 | GEO

Identification of cardiac developmental toxicants in differentiated human induced pluripotent stem cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-06-06 | GSE233926 | GEO

Transcriptomic characterization of C57BL/6 mouse embryonic stem cell differentiation and its modulation by developmental toxicants

Project description:To develop an in vitro model for developmental toxicity testing, we characterized gene expression changes during mouse embryonic stem cell (mESC) differentiation and their modulation by developmental toxicants. C57BL/6 mESCs were allowed to differentiate spontaneously and global gene expression changes were studied using microarrays at at different time points after embryoid body (EB) formation. Develpmental toxicants tested include 2.0 mM monobutyl phthalate (MBP), 0.25 mM thalidomide (THD), and 1.0 mM valproic acid (VPA).

2014-09-09 | GSE60174 | GEO

A transcriptome-based classifier to identify developmental toxicants by stem cell testing: design, validation, and optimization for histone deacetylase inhibitors

Project description:Test systems to identify developmental toxicants are urgently needed. A combination of human stem cell technology and transcriptome analysis was used here to provide proof-of-concept that toxicants with a related mode of action can be identified, and grouped for read-across. We chose a test system of developmental toxicity, related to the generation of neuroectoderm from pluripotent stem cells (UKN1), and exposed cells for six days to benchmark concentration (BMC) of histone deacetylase inhibitors (HDACi) valproic acid, trichostatin-A, vorinostat, belinostat, panobinostat and entinostat. To provide insight into their toxic action, we identified HDACi consensus genes, assigned them to superordinate biological processes, and mapped them to a human transcription factor network constructed from hundreds of transcriptome data sets. We also tested a heterogeneous group of ‘mercurials’ (methylmercury, thimerosal, mercury(II)chloride, mercury(II)bromide, 4-chloromercuribenzoic acid, phenylmercuric acid) (BMCs). Microarray data were compared at the highest non-cytotoxic concentration for all 12 toxicants. A support vector machine (SVM)-based classifier predicted all HDACi correctly. For validation, the classifier was applied to legacy data sets of HDACi, and for each exposure situation, the SVM predictions correlated with the developmental toxicity. Finally, optimization of the classifier based on 100 probe sets showed that eight genes (F2RL2, TFAP2B, EDNRA, FOXD3, SIX3, MT1E, ETS1, LHX2) are sufficient to separate HDACi from mercurials. Our data demonstrate, how human stem cells and transcriptome analysis can be combined for mechanistic grouping and prediction of toxicants. Extension of this concept to mechanisms beyond HDACi would allow prediction of human developmental toxicity hazard of unknown compounds with the UKN1 test system.

2015-07-21 | GSE71127 | GEO

Exosomal microRNAs secreted by induced pluripotent stem cell-derived cardiac cells

Project description:We report the secreted microRNAs from extracellular vesicles released from (i) cardiomyocytes, (ii) cardiac fibroblasts, (iii) endothelial cells differentiated from identical lines of normal human induced pluripotent stem cells and (iv) undifferentiated induced pluripotent stem cells.

2020-07-01 | GSE149290 | GEO

TCC in pluripotent and differentiated cells

Project description:TCC in pluripotent and differentiated cells

| PRJEB14689 | ENA

Classification of developmental toxicants in a human iPSC transcriptomics-based test

Project description:Classification of developmental toxicants in a human iPSC transcriptomics-based test

| PRJNA777253 | ENA