Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional characterization of ABCC1 and ABCB1 KO and MZ1 treated WT HAP1 cell lines.

ABSTRACT: Transcriptional characterization of ABCC1 and ABCB1 KO and MZ1 treated WT HAP1 cell lines.

PROVIDER: PRJNA979232 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR24807919_1.fastq.gz	Fastqsanger.gz
	SRR24807919_2.fastq.gz	Fastqsanger.gz
	SRR24807920_1.fastq.gz	Fastqsanger.gz
	SRR24807920_2.fastq.gz	Fastqsanger.gz
	SRR24807921_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 40

Similar Datasets

Transcriptional characterization of ABCC1 and ABCB1 KO and MZ1 treated WT HAP1 cell lines.

Project description:We used CRISPR/Cas9 to intoroduce frame-shifting mutations into the ABCC1 and ABCB1 genes and treated HAP1 WT cells with varying concentrations of the MZ1 PROTAC. To measure the transcriptional response to both genetic and chemical perturbances, we performed RNA-seq and quantified gene expression levels.

2023-06-07 | GSE234043 | GEO

Expression data from Hap1 parental control and Baz2B-KO cell lines

Project description:Baz2B protein was shown to form a chromatin remodelig complex mit Snf2H or Snf2L catalytic subunits. It is known that chromatin remodeling proteins regulate diffferent DNA-templated processes, therefore we wanted to know whether Baz2B plays a role in transcription regulation. We used microarrays to detail the global expression of transcripts in Hap1 parental cell line and compare them to Baz2B-KO. We found up- and down-regulated trasncripts that could be associated with the decreased cellular proliferation in Baz2B-KO cell line

2023-11-27 | GSE218705 | GEO

Gene expression analysis of human haploid cells (HAP1) depleted of SMARCB1 and SMARCA4

Project description:The aim of this study is to analyze the change in genome wide expression levels in HAP1 cells upon loss of SMARCB1, SMARCA4 or both these genes together. The SMARCB1 and SMARCA4 genes were the hits from a genome wide screen involving genetrap mutagenesis to find new players that are involved in sensitivity to Doxorubicin (Dox). It was found that loss of SMARCB1 and SMARCA4 genes impart resistance in HAP1 cells to Dox. To validate this, the genes were knocked out in HAP1 cells with CRISPR-Cas9 technology. Gene expression levels in SMARCB1 null, SMARCA4 null and SMARCB1-SMARCA4 double null cells were compared to wildtype HAP1 cells using RNAseq. From these experiments it was found that SMARCB1 loss caused several fold increase in ABCB1 gene levels. ABCB1 is an efflux pump in cells responsible for flushing out many small-molecule drugs. Further analysis of this gene confirmed that ABCB1 was the main factor responsible for Dox resistance upon SMARCB1 loss. In total there are four different cell types with two replicates for each cell type. Therefore, 8 samples in total.

2016-08-22 | E-GEOD-75515 | biostudies-arrayexpress

Gene expression analysis of human haploid cells (HAP1) depleted of SMARCB1 and SMARCA4

2016-08-22 | GSE75515 | GEO

LFQ shotgun proteomic analysis of HAP1 NatC KO cells

Project description:The impact of NatC subunits NAA30, NAA35 and NAA38 on the human proteome (protein abundance). Analysis of HAP1 WT, NAA30 KO, NAA35 KO and NAA38 KO cells

2023-09-20 | PXD034104 | Pride

RNA sequencing of HAP1 WT and MED13 KO clones

Project description:Purpose: The aim of the experiment was to eludicate differentialy expressed genes (DEGs) upon treatment with alkylating agent MMS between WT and MED13 deficient HAP1 cells. Results: After bioinformatic processing, at ≥2-fold change and an FDR≤0.1, 446 DEGs were identified in MED13 KO cells when compared to WT. Upon the MMS treatment 394 DEGs were identified in MED13 KO cells, of which 229 were common with untreated MED13 KO cells. Conclusions: Common DEGs represented genes directly regulated by MED13.

2020-12-29 | GSE147366 | GEO

Global transcriptome analysis of HAP1 cells

Project description:We analyzed the global change in transcription upon CHD3-KO and SENP1-KO compared to control HAP1 cells.

2018-08-05 | GSE111272 | GEO

ATAC sequencing of HAP1 cells

Project description:We analysed the global effect of CHD3-KO and SENP1-KO HAP1 cell lines compared to control cell line, on chromatin accessibility using ATAC-seq.

2018-08-05 | GSE111047 | GEO

LC-MSMS analysis of immunoprecipitated JunB-FLAG from HAP1 ADO KO cells

Project description:Define the N-terminal acetylation status of human JunB in siCTRL, siNAA10 or siNAA20 treated HAP1 ADO KO cells.

2024-07-03 | PXD045695 | Pride

Gene expression profiling of ABCB1 positive and ABCB1 negative OCM1A cells

Project description:Gene expression profiling of ABCB1 positive and ABCB1 negative OCM1A cells. ABCB1 is an ATP-dependent transporter efflux pump. OCM1A cell line is derived from uveal melanomas. 6 samples (3 ABCB1 positive and 3 ABCB1 negative) from 3 independent ABCB1 shift assays were analysed

2010-09-01 | E-GEOD-21425 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data