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RNA-seq of Recombinant Mouse Cold-inducible RNA-binding protein(rmCIRP)-treated Naïve CD4+ T cells

ABSTRACT: RNA-seq of Recombinant Mouse Cold-inducible RNA-binding protein(rmCIRP)-treated Naïve CD4+ T cells

PROVIDER: PRJNA979331 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24809154_1.fastq.gz	Fastqsanger.gz
	SRR24809154_2.fastq.gz	Fastqsanger.gz
	SRR24809155_1.fastq.gz	Fastqsanger.gz
	SRR24809155_2.fastq.gz	Fastqsanger.gz
	SRR24809156_1.fastq.gz	Fastqsanger.gz

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RNA-seq of Recombinant Mouse Cold-inducible RNA-binding protein(rmCIRP)-treated Naïve CD4+ T cells

Project description:Cold-inducible RNA-binding protein (CIRP) is a ubiquitous intracellular RNA chaperon, which serves as a damage-associated molecular pattern (DAMP) when released outside the cells upon stimulation by inflammation and infection. It has been shown that CIRP promotes splenic CD4+ and CD8+ T-cell activation in vivo and predisposes CD4+ T cells to a Th1 hyperinflammatory response profile in vitro. However, the effect and underlying mechanisms of CIRP on Th17/Treg differentiation is still unclear. This study reveals the transcriptomic changes of mouse Naïve CD4+ T cells induced by Recombinant Mouse CIRP (rmCIRP) in vitro.

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Project description:In mammalian tissues circadian gene expression can be driven by local oscillators or systemic signals controlled by the master pacemaker in the suprachiasmatic nucleus. Here we show that simulated body temperature cycles, but not peripheral oscillators, can control the rhythmic expression of Cold-Inducible RNA binding Protein (CIRP) in cultured fibroblasts. In turn, loss-of-function experiments indicate that CIRP is required for high amplitude circadian gene expression. The transcriptome-wide identification of CIRP-bound RNAs by a biotin-streptavidin based CLIP-seq procedure revealed several CIRP-bound transcripts encoding circadian oscillator proteins. One of these, CLOCK, accumulated to particularly low levels in CIRP-depleted fibroblasts. Since ectopic expression of CLOCK improved circadian gene expression in these cells, we surmise that CIRP confers robustness to circadian oscillators via the regulation of CLOCK expression. Identification of CIRP-interacting RNA molecules in NIH3T3 cells and RNA expression in NIH3T3 cells treated with Control or CIRP siRNA after incubation of the cells at 33M-BM-0C for 8 hours

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