Project description:Whole-genome analysis by 62-strain microarray showed variation in resistance and virulence genes on mobile genetic elements (MGEs) between 40 isolates of methicillin-resistant Staphylococcus aureus (MRSA) strain CC22-SCCmecIV but also showed (i) detection of two previously unrecognized MRSA transmission events and (ii) that 7/8 patients were infected with a variant of their own colonizing isolate. [Data is also available from http://bugs.sgul.ac.uk/E-BUGS-128]
Project description:The study aims to characterize a new isolate that lacks mecA but is nevertheless resistant to methicillin in order to find a novel mechanism of methicillin resistance.
Project description:Methicillin-resistant Staphylococcus aureus is one of the major causative agents associated to infections with a high morbidity and mortality in hospitals worldwide. In previous studies, we reported that lignan 3'-demethoxy-6-O-demethylisoguaiacin isolated and characterized from Larrea tridentata showed the best activity towards methicillin-resistant S. aureus. Understanding of mechanism of action of drugs allows design drugs in a better way. Therefore, we employed microarray to obtain gene expression profile of methicillin-resistant S. aureus after exposure to 3'-demethoxy-6-O-demethylisoguaiacin. The results showed that lignan had an effect on cell membrane affecting proteins of the ATP-binding cassette (ABC) transport system causing bacteria death.
Project description:The study aims to characterize a new isolate that lacks mecA but is nevertheless resistant to methicillin in order to find a novel mechanism of methicillin resistance. Samples were hybridized on aminosilane coated slides with 70-mer oligos.
Project description:Methicillin-resistant Staphylococcus aureus (MRSA) infections result in more than 200,000 hospitalizations and 10,000 deaths in the United States each year and remain an important medical challenge. To better understand the transcriptome of Staphylococcus aureus USA300 NRS384, a community-acquired MRSA strain, we have conducted an RNA-Seq experiment on WT samples.
Project description:Methicillin-resistant Staphylococcus aureus (MRSA) strains are important human pathogens and a significant health hazard for hospitals and the food industry. They are resistant to β-lactam antibiotics including methicillin and extremely difficult to treat. In this study, we show that the Staphylococcus aureus COL (MRSA) strain, with a known complete genome, can easily survive and grow under acidic and alkaline conditions (pH 5 and pH9, respectively), both planktonically and as a biofilm. Α microarray-based analysis of both planktonic and biofilm cells was performed under acidic and alkaline conditions showing that several genes are up- or down-regulated under different environmental conditions and growth modes. These genes were coding for transcription regulators, ion transporters, cell wall biosynthetic enzymes, autolytic enzymes, adhesion proteins and antibiotic resistance factors, most of which are associated with biofilm formation. These results will facilitate a better understanding of the physiological adjustments occurring in biofilm-associated S. aureus COL cells growing in acidic or alkaline environments, which will enable the development of new efficient treatment or disinfection strategies. We used microarrays to detail the global programme of gene expression underlying growth of S. aureus COL growing under acidic and alkaline conditions in biofilm or planktonic mode and identified distinct classes of up-regulated genes during this process.
Project description:Methicillin-resistant Staphylococcus aureus (MRSA) is a highly prioritized pathogen proposed by World Health Organization (WHO). It is listed to encourage researchers to search for effective antimicrobial agents. Previously, we isolated soil Brevibacillus sp. strain SPR19, which showed anti-MRSA activity. However, the active substances were still unknown. This study aimed to isolate and identify the anti-MRSA substances from this bacterium. The cell-free supernatant of SPR19 was subjected to salt precipitation, cation exchange, and re-versed-phase chromatography. Bioassay-guided fractionation was used to screen active sub-stances
