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Long Non-Coding RNA MEG3 Promotes Angiogenesis by directly regulating integrin signaling impairing endothelial cell function.

ABSTRACT: Long Non-Coding RNA MEG3 Promotes Angiogenesis by directly regulating integrin signaling impairing endothelial cell function.

PROVIDER: PRJNA981989 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24882366_1.fastq.gz	Fastqsanger.gz
	SRR24882366_2.fastq.gz	Fastqsanger.gz
	SRR24882367_1.fastq.gz	Fastqsanger.gz
	SRR24882367_2.fastq.gz	Fastqsanger.gz
	SRR24882368_1.fastq.gz	Fastqsanger.gz

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Long non-coding RNA MEG3 promotes angiogenesis by directly regulating integrin signaling impairing endothelial cell function

Project description:Long non-coding RNA MEG3 promotes angiogenesis by directly regulating integrin signaling impairing endothelial cell function

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Long Non-Coding RNA MEG3 Promotes Angiogenesis by directly regulating integrin signaling impairing endothelial cell function [ChIRP-seq]

Project description:Long Non-Coding RNA MEG3 Promotes Angiogenesis by directly regulating integrin signaling impairing endothelial cell function [ChIRP-seq]

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Long Non-Coding RNA MEG3 Promotes Angiogenesis by directly regulating integrin signaling impairing endothelial cell function [ChIP-seq]

Project description:Long Non-Coding RNA MEG3 Promotes Angiogenesis by directly regulating integrin signaling impairing endothelial cell function [ChIP-seq]

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Long non-coding RNA MEG3 promotes angiogenesis by directly regulating integrin signaling impairing endothelial cell function

Project description:We depleted MEG3-lncRNA using GapmeRs in primary human ECs. Total MEG3-regulated targets were obtained.

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Long Non-Coding RNA MEG3 Promotes Angiogenesis by directly regulating integrin signaling impairing endothelial cell function [ChIP-seq]

Project description:We immunoprecipitated EZH2, together with associated chromatin isolated from the HUVECs (2 x 10^6) that were transfected with MEG3 GapmeRs (10 nM, 48 h) or a scrambled control GapmeRs (Ctr). For transfection, we used 10 nM scrambled LNA (locked nucleic acids) GapmeR control (Cat. No. 339515) or phosphorothioate antisense standard GapmeRs MEG3-lncRNA (Cat No. 339511, Qiagen). On beads crosslinked chromatin complexes were reversed, and DNA purified using QIAquick® PCR Purification Kit and quantified by Qubit HS assay (Q33230)

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Long Non-Coding RNA MEG3 Promotes Angiogenesis by directly regulating integrin signaling impairing endothelial cell function [ChIRP-seq]

Project description:We profiled MEG-DNA interactions using gluteraldehyde assisted chromatin immunoprecipitation by RNA purification (ChIRP-seq) in primary human ECs. Genome-wide MEG3-associated loci were obtained.

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Histone H3K27 methyltransferase EZH2 interacts with MEG3-lncRNA to directly regulate integrin signaling and endothelial cell function

Project description:Histone H3K27 methyltransferase EZH2 interacts with MEG3-lncRNA to directly regulate integrin signaling and endothelial cell function

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Long Non-Coding RNA MEG3 Promotes Angiogenesis by directly regulating integrin signaling impairing endothelial cell function.

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-04-12 | GSE234544 | GEO

Integrin αvβ3 acting as membrane receptor for thyroid hormones mediates angiogenesis in malignant T cells

2015-02-18 | GSE63122 | GEO

Integrin αvβ3 acting as membrane receptor for thyroid hormones mediates angiogenesis in malignant T cells

Project description:We have found that thyroid hormones (THs), acting as soluble integrin αvβ3 ligands, activate growth-related signaling pathways in T-cell lymphomas (TCL). Specifically, TH-activated αvβ3 integrin signaling promotes TCL proliferation and angiogenesis, in part, via the up-regulation of VEGF. CUTLL1 cells were treated with T3- and T4-bound agarose or agarose alone for 24hrs. Total RNA was harvested from cells and used for expression profiling via RNA-seq.

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