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Differential gene expression in HCC cell lines Huh7 and PLC under high and low glucose culturement

ABSTRACT: Differential gene expression in HCC cell lines Huh7 and PLC under high and low glucose culturement

PROVIDER: PRJNA983704 | ENA |

REPOSITORIES: ENA

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Differential gene expression in HCC cell lines Huh7 and PLC under high and low glucose culturement

Project description:To gain a deeper understanding of the mechanism by lncRNAs involved in glucose metabolism during the progression of HCC, we conducted transcriptome sequencing on HCC cells treated with low (5 mM) and high (25 mM) glucose and carried out analyses for the lncRNAs with a significant change of expression combined with TCGA-LIHC data. Huh7 and PLC cells were treated with low glucose (5mM) or high glucose (25mM) for 48 h, whole-transcriptome sequencing (RNA-Seq) was performed to identify the significant changed lncRNAs with glucose.

2024-11-24 | GSE234898 | GEO

The RNA-seq of HCC specimens and the RIP-seq of HNRNPD in PLC cells and WT (DMSO or Compound C) and circLARP1B-Def (DMSO) PLC/PRF/5 cells

Project description:To investigate functional transcripts in metastatic HCC, we performed high-throughput RNA sequencing (RNA-seq) of tumors from 3 metastatic HCC and 3 non-metastatic HCC. And we performed RIP-seq human PLC/PRF/5 cells to investigate the HNRNPD binding transcripts. To investigate function of circLARP1B on AMPK pathway, we performed high-throughput RNA sequencing (RNA-seq) of WT (DMSO or Compound C) and circLARP1B-Def (DMSO) PLC/PRF/5 cells.

2023-04-02 | GSE217403 | GEO

HCC Cell lines: MHCC97L vs. Huh7 and HCCLM3

Project description:To identify genes associated with HCC invasiveness and metastasis, a hybridization-based microarray assay was used to analyze three cell lines with increasing metastatic potentials, Huh7,MHCC97L, and HCCLM3

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Expression data comparing Huh7 CD133+ and CD133- HCC cells

Project description:To identify candidate genes involved in enhanced tumorigenicity of CD133+ liver tumor-initiating cells Affymetrix Human Genome U133 Plus GeneChip 2.0 HCC cell line Huh7 was sorted into CD133+ and CD133- populations by flow cytometry

2012-04-04 | E-GEOD-23450 | biostudies-arrayexpress

MeRIP-seq analysis for CSTF2 knockout in HUH7 and PLC/PRF/5 cell lines

Project description:CSTF2 has been shown to have a certain oncogenic effect in hepatocellular carcinoma, but its mechanism remains unclear. Previous studies have suggested that CSTF2 can shorten the 3' untranslated region (3'UTR) of target genes. Considering that the 3'UTR contains numerous m6A modification sites, we hypothesize that CSTF2 may regulate mRNA m6A modification. We performed MeRIP-seq analysis to investigate the changes in m6A modification in CSTF2 knockout HUH7 and PLC/PRF/5 cell lines.

2024-05-30 | GSE268346 | GEO

Glucose deprivation effect on Hepatocellular carcinoma cell line: Huh-7

Project description:Analysis of Hepatocellular carcinoma (HCC) cell line - Huh7 deprived of glucose. Glucose restriction promotes a liver tumor-initating cell phenotype. Results provide insight into the molecular mechanism of glucose deprivation in HCC.

2024-06-17 | GSE152927 | GEO

Investigation of downstream genes regulated by ACOT12 in HCC cells [Huh7]

Project description:Huh7 cell, a human hepatocellular carcinoma (HCC) cell line, was stably overexpressed with scramble shRNA or shRNA against ACOT12. These cells were then used for gene expression profiles analysis.

2019-01-01 | GSE111760 | GEO

Effect of depletion or overexpression of EGR1 on gene expression in HCC cells (deletion in MHCC97H cells, overexpression in PLC/PRF5 cells)

Project description:To investigate the function of EGR1 in HCC growth, we established EGR1 knock out MHCC97H cells by CRISPR/Cas9 system and EGR1-overexpressing PLC/PRF5 cells. We then performed gene expression profiling analysis using data obtained from RNA-seq of EGR1 knock out MHCC97H cells and parental MHCC97H cells, EGR1-overexpressing PLC/PRF5 cells and control PLC/PRF5 cells.

2024-02-07 | GSE238116 | GEO

Proteome of Human hepatocellular carcinoma cell lines

Project description:The proteomic profiling of nine commonly used HCC cell lines Hep3B, HepG2, HepG2.2.15, HUH7, PLC/PRF/5, MHCC97L,MHCC97H,HCCLM3 and HCCLM6

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Expression data from CSC and Non-CSC of three HCC cell lines

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