Eisenia fetida
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA98375 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Profiling differentially expressed earthworm genes in response to TNT, RDX, or TNT+RDX exposure.
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Project description:Discovery of biomarker genes form earthworm microarray data
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Project description:Microarray analysis of CL-20 reversible neurotoxicity in the earthworm Eisenia fetida
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Project description:Earthworm (Eisenia fetida) microRNA discovery from small RNAs isolated from whole body tissue
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Project description:The earthworm Eisenia fetida is one of the most used species in standardized soil ecotoxicity tests. Endpoints such as survival, growth and reproduction are ecologically relevant but provide little mechanistic insight into the toxicity pathways, especially at the molecular level. To better understand toxicological modes of action and to facilitate the development of molecular biomarkers, we have obtained 30,245 unique EST sequences from E. fetida and have designed a novel microarray with 15,119 60-mer oligonucleotide probes. These probes target the unique non-redundant EST sequences identified in E. fetida. Using this array we have profiled gene expression of E. fetida after exposure to CL-20, a cage cyclic nitramine previously found exhibiting reversible neurotoxicity to worms. Worms were exposed for 6 days to CL-20. Half of the exposed worms were allowed to recover in a clean environment for 7 days. Electrophysiological analysis showed that the conduction velocity of worm medial giant nerve fiber was significantly decreased after 6-d exposure to CL-20, and that giant nerve fiber function was restored at the end of the 7-d recovery period. Total RNA samples isolated from four treatment groups (6 replicates per group), i.e., 6-d control, 6-d exposed, 13-d control and 6-d exposed with 7-d recovery, were analyzed using the new 15K oligo array. Bioinformatics and statistical analyses have identified specific neurological pathways affected by CL-20 and recovery of these pathways after CL-20 removal. These results provide significant insights on the CL-20 toxic mode of action and how earthworms can recover from chemical stressors.
2009-10-14 | GSE18536 | GEO
Project description:To understand molecular mechanisms of the chronic, sublethal toxicity of 2,4,6-trinitrotoluene (TNT), a widely used ordnance compound of public concerns, we constructed a microarray consisting of 4,032 cDNA isolated from the earthworm Eisenia fetida using the suppressive subtractive hybridization technique. Worms were exposed to a gradient of TNT-spiked soil for 28 days. Based on the reproduction response to TNT, four treatments, i.e., control, 7, 35 and 139 ppm, were selected for gene expression studies. Keywords: Sublethal toxicity of TNT (dose-response) to earthworm (Eisenia fetida)
2007-10-15 | GSE7024 | GEO
Project description:Using pooled earthworm RNA to test 244K probes on TA-2 test array design 2520023 antisense 1
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Project description:Using pooled earthworm RNA to test 244K probes on TA-3 test array design 2520024 sense 2
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Project description:Using pooled earthworm RNA to test 244K probes on TA-1 test array design 2520022 sense 1
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Project description:Using pooled earthworm RNA to test 244K probes on TA-4 test array design 2520025 antisense 2
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