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Genomic profiling of striatum from a mouse model of Huntington's disease (Q140) crossed with Msh3 knockout mice [ATACSeq]

ABSTRACT: Genomic profiling of striatum from a mouse model of Huntington's disease (Q140) crossed with Msh3 knockout mice [ATACSeq]

PROVIDER: PRJNA985665 | ENA |

REPOSITORIES: ENA

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Genomic profiling of striatum from a mouse model of Huntington's disease (Q140) crossed with Msh3 knockout mice [ATACSeq]

Project description:Striatum was isolated from 12-month-old Msh3-/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Genomic analysis (ATACseq) was performed on 4 genotypes: WT, Msh3-/-, Het (Q140) KI, and Het (Q140) KI X Msh3-/-, 6 replicates per genotype.

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Project description:As part of collaboration between UCLA and CHDI, UCLA is creating knockouts (KOs) of 123 genes, implicated in Huntington’s disease (HD) through various computational modeling efforts. Striatum was isolated from 12-month-old Msh3+/- mice and Msh3-/- (KO) crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 6 genotypes: WT, Msh3+/-, Msh3-/-, Q140, Q140 X Msh3+/- and Q140 X Msh3-/-, 8 replicates per genotype.

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Project description:Transcriptional profiling of striatum from a mouse model of Huntington's disease (Q140) crossed with Msh3 heterozygous and homozygous mice [Msh3 snRNA-seq]

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