Ontology highlight
ABSTRACT:
PROVIDER: PRJNA986113 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR24981143_1.fastq.gz | Fastqsanger.gz | |||
| SRR24981143_2.fastq.gz | Fastqsanger.gz | |||
| SRR24981144_1.fastq.gz | Fastqsanger.gz | |||
| SRR24981144_2.fastq.gz | Fastqsanger.gz | |||
| SRR24981145_1.fastq.gz | Fastqsanger.gz |
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Project description:Here we have compared adult wildtype (N2) C. elegans gene expression when grown on different bacterial environments/fod sources in an effort to model naturally occuring nematode-bacteria interactions at the Konza Prairie. We hypothesize that human-induced changes to natural environments, such as the addition of nitrogen fertalizer, have effects on the bacterial community in soils and this drives downstream changes in the structure on soil bacterial-feeding nematode community structure. Here we have used transcriptional profiling to identify candidate genes involved in the interaction of nematodes and bacteria in nature.
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Project description:Entomopathogenic nematodes, the beneficial soil-dwelling nematodes identified with noteworthy ecological services of insect pest management naturally, harbor and vector obligate bacterial symbionts which together cause rapid insect mortality. The tritrophism among the bacterium-nematode-insect host is a treasure box of biological, molecular and biochemical information that was examined to a limited extent. In this project, we intend to explore the time-course expression of proteins of test insect, Holotrichia serrata larvae, during the progression of larval mortality due to nematode-bacterium infection, more specifically, Heterorhabditis indica-
Photorhabdus luminescens, Steinernema spp. vs Xenorhabdus spp., respectively. The data submitted relates to the insect protein profiles (LC-MSMS) in healthy, infected and morbid, and dead larvae of Holotrichia serrata.
2025-01-10 | PXD048306 | JPOST Repository
Project description:Entomopathogenic nematodes, the beneficial soil-dwelling nematodes identified with noteworthy ecological services of insect pest management, naturally, harbor and vector obligate bacterial symbionts which together cause rapid insect mortality. The tritrophism among the bacterium-nematode-insect host is a treasure box of biological, molecular and biochemical information that was examined to a limited extent in other studies. We have, herein, aimed at quantifying the concentration and at generating the total proteomic profiles of one insect host, Spodoptera frungiperda, during the progression of infection with 4 EPN and their associated bacteria, as compared to that of healthy controls. The time-course expression of larval proteins of the test insect, Spodoptera frugiperda, during the progression of larval mortality due to nematode-bacterium infection, more specifically,4 different combinations of Heterorhabditis indica-Photorhabdus luminescens, Steinernema spp. vs Xenorhabdus spp., respectively. The proteomic profiling was facilitated through LC-MS/MS. The data submitted relates to the insect protein profiles (LC-MSMS) in healthy, infected and morbid, and dead larvae of Spodoptera frugiperda.
2025-01-13 | PXD048520 | JPOST Repository
Project description:89 small non-coding RNAs (ncRNAs) were identified in the soil-dwelling alpha-proteobacterium Rhizobium etli by comparing an extensive compilation of ncRNA predictions to intergenic expression data of a whole-genome tiling array. The differential expression levels of some of these ncRNAs during free-living growth and during interaction with the eukaryotic host plant may indicate a role in adaptation to changing environmental conditions.
2009-11-17 | GSE18580 | GEO
Project description:Temperature is an important ecological condition, and sudden temperature changes in soil can induce stress in soil-dwelling invertebrates. Soil animals can move to more favorable habitats and/or adapt physiologically to a stressful environment. Hyperthermic conditions will impact gene expression as one of the first steps. We use a transcriptomics approach to identify the transcripts of which expression changed in response to heat stress in the springtail Folsomia candida using a 5,131 probe microarray. A temperature shift from 20°C to 30°C for 30 minutes significantly altered the expression of 142 genes, of which 116 were upregulated, and 26 downregulated. Many upregulated genes encoded heat shock proteins (Hsps) or enzymes involved in the synthesis of ATP, such as members of the electron transport chain. Furthermore, genes involved in oxidative stress and anion-transporting ATPases were upregulated. Downregulated were glycoside hydrolases, involved in catalysis of certain disaccharides, which indicate an accumulation of stress-protective disaccharides. The microarray results from this study, which were validated using quantitative RT PCR, reveal a mild response to heat shock in this soil invertebrate, relative to other organisms. This may be due to specific ecological factors during evolution of soil invertebrates, such as the relatively stable temperatures in the soil habitat. This study presents potential candidate genes for future functional studies concerning thermal stress in soil-dwelling invertebrates, like e.g., the investigation of the heat hardening process.
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Project description:Indole is an intercellular and interkingdom signaling molecule, which is widespread in diverse ecological niches. Caenorhabditis elegans is a bacterivorous nematode living in soil and compost environments and a useful model host for the study of host-microbe interactions. While various bacteria and some plants produce a large quantity of extracellular indole, little is known about the effects of indole, its derivatives, and indole-producing bacteria on behaviors in C. elegans and animals. Here, we show that C. elegans senses and moves toward indole and indole-producing bacteria, such as Escherichia coli, Shigella boydii, Providencia stuartii, and Klebsiella oxytoca, while avoids non-indole producing pathogenic bacteria. It was also found that indole-producing bacteria and non-indole-producing bacteria exert divergent effects on egg-laying behavior of C. elegans via indole. In addition, various indole derivatives also modulate chemotaxis, egg-laying behavior, and survival of C. elegans. In contrast, indole at a high concentration to kill C. elegans that has the ability to detoxify indole via oxidation and glucosylation, indicating predator-prey interactions via a double-edged molecule indole. Transcriptional analysis showed that indole markedly up-regulated gene expression of cytochrome P450 family, UDP-glucuronosyltransferase, glutathione S-transferase, which explained well the modification of indole in C. elegans, while down-regulated expression of collagen genes and F-box genes. Our findings suggest that indole and its derivatives are important interkingdom signaling molecules in bacteria-nematode interactions.
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