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The ALOG domain defines a new family of plant-specific transcription factors acting during Arabidopsis flower development

ABSTRACT: The ALOG domain defines a new family of plant-specific transcription factors acting during Arabidopsis flower development

PROVIDER: PRJNA986886 | ENA |

REPOSITORIES: ENA

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The ALOG domain defines a new family of plant-specific Transcription Factors acting during Arabidopsis flower development.

Project description:The ALOG domain defines a new family of plant-specific Transcription Factors acting during Arabidopsis flower development.

| PRJNA1037151 | ENA

The ALOG domain defines a new family of plant-specific transcription factors acting during Arabidopsis flower development

Project description:The ALOGs (Arabidopsis LIGHT-DEPENDENT SHORT HYPOCOTYLS 1 and Oryza G1) are Transcription Factors (TFs) from an evolutionarily conserved plant-specific family shown to play critical roles in meristem identity, inflorescence architecture and organ boundaries in diverse species from mosses to higher flowering plants. However, the DNA binding-specificity and molecular determinants of protein-DNA interactions of this family were uncharacterized. Using in vitro genome-wide studies, we identified the conserved DNA motif bound by ALOG proteins from the liverwort Marchantia polymorpha and the flowering plants Arabidopsis, tomato and rice. In order to determine the amino acids responsible for DNA-binding specificity, we solved the 2.1Å structure of the ALOG DNA binding domain in complex with its cognate DNA. The ALOG DBD is an all-alpha helical domain with a structural zinc ribbon insertion and an N-terminal disordered NLS. The NLS sequence forms an integral part of the DNA binding domain and contributes to direct base read-out. To define the function of a group of redundant ALOG proteins in the model plant Arabidopsis thaliana, we generated a series of alog mutants and uncovered their participation in a gene regulatory network involving the other floral regulators LEAFY, BLADE-ON-PETIOLE and PUCHI, all active in defining boundary regions between reproductive meristems and repressing bracts development. Taken together, this work provides the biochemical and structural basis for DNA-binding specificity of an evolutionarily conserved TF family and reveals its role as a key player in defining organ boundaries in Arabidopsis.

2024-02-06 | GSE235674 | GEO

The ALOG domain defines a new family of plant-specific Transcription Factors acting during Arabidopsis flower development.

Project description:Analysis of the transcriptome of the reproductive meristems of Arabidopsis thaliana Col-0. We compared the transcriptome obtained from lsh1 lsh3 lsh4 triple mutant to a wild type control

2024-02-06 | GSE247357 | GEO

Pilot Experiment for Cell-specific Translatome: AP1 Domain vs. Whole Flower

Project description:A pilot experiment for an RNA-seq dataset (SRP003234). This experiment compares AP1 domain-specific translating RNA with total flower translating mRNA. Two-condition experiment, AP1 flower domains vs. whole flower at flower stage 4.

2010-08-25 | E-GEOD-23777 | biostudies-arrayexpress

Pilot Experiment for Cell-specific Translatome: AP1 Domain vs. Whole Flower

Project description:A pilot experiment for an RNA-seq dataset (SRP003234). This experiment compares AP1 domain-specific translating RNA with total flower translating mRNA.

2010-08-25 | GSE23777 | GEO

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Project description:Chemotyping dataset of n-butanol peptide extract of Chenopodium quinoa flower

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Reversible flower opening regulated by CPK16-dependent phosphorylation and activation of aquaporin PIP2;2 in Gentiana

Project description:Flower opening is important for successful pollination in many plant species, and some species repeat reversible flower opening and closing movements. This is thought to be due to the turgor pressure change caused by the water influx/efflux, which depends on osmotic oscillation in the cells. In some ornamental plants, it has been suggested that water channel aquaporin may play an important role in flower opening. However, the molecular mechanism(s) involved in flower movement are largely unknown. Using Gentiana flowers, which show reversible movement in response to temperature and light stimuli, as a model, we showed that reversible flower opening is regulated by aquaporin GsPIP2;2 and GsPIP2;7. In particular, phosphorylation of the C-terminal serine residue of GsPIP2;2 is important for the transport activity and correlates closely with the flower re-opening rate. Furthermore, GsPIP2;2 is phosphorylated and activated by GsCPK16, which is activated by elevated cytosolic Ca2+ levels in response to temperature and light stimuli. We propose that reversible flower opening is regulated by GsCPK16-dependent GsPIP2;2 phosphorylation and activation, with stimulus-induced calcium signals acting as triggers. CPK-dependent phosphorylation and activation of PIP2s may be one of the universal regulatory mechanisms for flower opening in plants.

2022-10-17 | PXD029169 | JPOST Repository

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