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Yeast beta-glucan diets alter bone-marrow derived macrophage function

ABSTRACT: Yeast beta-glucan diets alter bone-marrow derived macrophage function

PROVIDER: PRJNA986912 | ENA |

REPOSITORIES: ENA

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Yeast beta-glucan diets alter bone-marrow derived macrophage function

Project description:Fungal beta-glucans are major drivers of trained immunity which increases long-term protection against secondary infections. Heterogeneity in beta-glucan source, structure and solubility alters interaction with the phagocytic receptor Dectin-1 and could impact strategies to improve trained immunity in humans. Using a panel of diverse beta-glucans we describe the ability of a specific yeast-derived whole-glucan particle (WGP) to reprogramme metabolism and thereby drive trained immunity in human monocyte-derived macrophages in-vitro and mice bone-marrow in-vivo. Presentation of pure, non-soluble, non-aggregated WGPs led to the formation of the Dectin-1 phagocytic synapse with subsequent lysosomal mTOR activation, metabolic reprogramming and epigenetic rewiring. Intraperitoneal or oral administration of WGP drove bone-marrow myelopoiesis and improved mature macrophage responses, pointing to therapeutic and food-based strategies to drive immune training. Thus, the investment of a cell in a trained response relies on specific recognition of beta-glucans presented on intact microbial particles through stimulation of the Dectin-1 phagocytic response.

2023-09-21 | GSE235691 | GEO

RNASeq of bone marrow-derived macrophages treated with beta-glucan and lipopolysaccharide (LPS)

Project description:We found that beta-glucan treatment induces early induction of transcripts associated with inflammation and metabolism. Several days after beta-glucan treatment, genes associated with differentation and housekeeping remain upregulated. LPS treatment induces a strong inflammatory response. Beta-glucan did not significantly alter the macrophage response to LPS.

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How different diets alter skeletal muscle in pig

2005-08-31 | GSE2785 | GEO

How different diets alter skeletal muscle in pig

Project description:we want to test how different diets (high energy diet HED and low energy diet LED) alter muscle methabolism in pigs. we perform different array experiments using an human platform (GPL2011) and RNA extracted from pig skelethal muscle. thank's this we also test cross-species hybridisation. extraction of RNA from pig skeletal muscle by a modification of Chomczynski protocol. cDNA was purified and labelled with Cy3 and Cy5 fluorochromes using the cDNA labeling purification module kit (Invitrogen). The HED samples were analysed in comparison to LED samples, used as reference. The labelled cDNA were appropriately coupled and used for competitive hybridisation on the same microarray at 42Â°C for 16h. The relative intensity of labelled cDNA in HED and LED was acquired with ScanArray LITE scanner (PerkinElmer Life Sciences, Inc). platform used for our experiments is GPL2011.

2010-06-09 | E-GEOD-2785 | biostudies-arrayexpress

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Project description:Induction of trained immunity by beta-glucan affects myeloid cells and their bone marrow progenitors. In particular, broad alterations in the transcriptome of trained myeloid cells have been demonstrated. In this study, we performed RNA sequencing in GMP from beta-glucan treated mice, as compared to control-treated mice, in order to investigate the impact of trained immunity on the transcriptomic profile of GMP.

2020-10-30 | GSE139452 | GEO

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Project description:glucan Raw sequence reads

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The effect of beta-glucan - induced trained immunity on the epigenetic profile of bone marrow granulocyte-macrophage progenitors (GMP) and neutrophils

Project description:Induction of trained immunity by beta-glucan affects myeloid cells and their bone marrow progenitors. In particular, broad epigenetic alterations in trained myeloid cells have been demonstrated. In this study, we performed single cell ATAC sequencing in GMP and neutrophils from beta-glucan treated mice, as compared to control-treated mice, in order to investigate the impact of trained immunity on the epigenetic profile of GMP and neutrophils.

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Transcrioptome analysis of human primary macrophages stimulated by bacterial endotoxin lipopolysaccharide (LPS), and (1,3)-b-glucans, curdlan and glucan from bakerM-BM-4s yeast (GBY)

Project description:We performed gene expression microarray experiments to compare the global transcriptional response induced by b-glucans and LPS with their secretomes. We identified 1683, 767 and 1447 genes with over two-fold increase or decrease in LPS-, curdlan- or GBY-stimulated macrophages, respectively. We show that both LPS and b-glucan induces significant gene expression changes in macrophages, but only b-glucans activate a robust protein secretion. The gene expression of human primary macrophage cells was measured at 6 hours after exposure the cells to 1 M-BM-5g LPS or 10 M-BM-5g Curdlan or 100 M-BM-5g GBY (glucan from bakerM-BM-4s yeast), also 0-group was included. Three independent experiments were performed using three different donors for each experiment.

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Genome-wide analysis of mDC transcriptional response to cyclic glucan

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