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Expression analysis of C. elegans DAF-15 neuronal AID worms

ABSTRACT: Expression analysis of C. elegans DAF-15 neuronal AID worms

PROVIDER: PRJNA988074 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25040720_1.fastq.gz	Fastqsanger.gz
	SRR25040720_2.fastq.gz	Fastqsanger.gz
	SRR25040721_1.fastq.gz	Fastqsanger.gz
	SRR25040721_2.fastq.gz	Fastqsanger.gz
	SRR25040722_1.fastq.gz	Fastqsanger.gz

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Expression analysis of C. elegans DAF-15 neuronal AID worms

Project description:Investigation of whole genome gene expression level changes in C. elegans DAF-15 neuronal AID worms under 5-Ph-IAA treatment. This project contains 8 samples (4 treated by etoh and 4 treated by 5-Ph-IAA) of RNA-seq data.

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Project description:To identify changes in gene expression in daf-16 mutants, we compared expression in fer-15 young adults to expression in fer-15(b26); daf-16(m26) young adults grown at 25?C. At Stanford University, four samples of fer-15 and five samples of fer-15; daf-16 animals were prepared. At the University of Colorado, seven samples of fer-15 and six samples of fer-15; daf-16 was prepared. Set of arrays that are part of repeated experiments Place of growth: At Stanford vs Colorado Genotype: mutant strains vs wt (N2) mixed stage; some expts are reverse dye (wt in channel 2)

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Project description:Under adverse environmental conditions, nematodes arrest into dauer, an alternative developmental stage for diapause. Dauers endure unfavorable environment and interact with host animals to access favorable environments, thus playing a critical role in the survival of both free-living and parasitic nematodes. Here, we discovered that in Caenorhabditis elegans, daf-42 is essential for development into dauer stage, as the null mutant shows lethal phenotype during dauer entry. To examine the transcriptional changes accompanied by the absence of DAF-42 during dauer entry, we performed RNA-sequencing on daf-2 and daf-2; daf-42 worms at 52 hours after egg laying (HAE) and 60 HAE, the time at which 0% and about 40% of daf-2; daf-42 mutants form dead dauer at 25 degrees Celcius, respectively. daf-2 control worms develop into dauer stage after 60 HAE, and half of its population develop into dauer by 72 HAE, when raised at 25 degrees Celcius.

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