Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Glycine max cultivar:Richland (PI 548406) yellow cultivar with dominant I allele

ABSTRACT: Glycine max cultivar:Richland (PI 548406) yellow cultivar with dominant I allele Genome sequencing

PROVIDER: PRJNA989342 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Glycine max cultivar:PI 594527

Project description:Glycine max cultivar:PI 594527 Raw sequencing reads and assembly

| PRJNA718574 | ENA

Project description:Glycine max (Richland, UC41, I allele) genome resequencing

| PRJNA503615 | ENA

Allele-Specific gene editing approach for a dominant form of Epidermolysis Bullosa Simplex I

Project description:Epidermolysis Bullosa Simplex (EBS) is the most common form of Epidermolysis Bullosa (EB) and it is mainly inherited in an autosomal dominant manner (prevalence 1/30000 – 1/50000). Several clinical variants have been described based on the mutated gene, the site of blister formation and the anatomical distribution, but the vast majority of the patients display dominant mutations in genes encoding keratin 5 (KRT5) and keratin 14 (KRT14). The lack of functional keratin intermediate filaments causes basal keratinocytes to exhibit a dramatic cytoplasmatic softening and rupture, when subjected to minor mechanical traction, leading to the distinctive EBS patients intraepidermal blisters formation. Whilst viral mediated addition of a corrected copy of the altered gene is the ascertained approach to tackle recessively inherited EB (such as Junctional and Dystrophic EB), a potential successful combined cell and gene therapy for EBS dominant forms requires the editing of the mutated gene. In this case study, we outlined an allele specific CRISPR/Cas9 gene editing approach able to specifically detect and disrupt a de novo monoallelic c.475/495del21 mutation within exon 1 of KRT14. Taking advantage of the tailored CRISPR/Cas9 system to induce a NHEJ mediated frameshift mutations introduction, we attained a remarkable mutant allele knock-out efficiency. Following KRT14 mutant allele specific gene editing, patient derived primary keratinocytes (EBS01) restored a normal intermediate filament network and mechanical stress resilience.

2023-12-06 | GSE221645 | GEO

Allele-Specific gene editing approach for a dominant form of Epidermolysis Bullosa Simplex II

2023-12-06 | GSE246343 | GEO

Glycine max strain:Fiskeby III | cultivar:PI 438471

Project description:Glycine max strain:Fiskeby III | cultivar:PI 438471 Genome sequencing

| PRJNA1107527 | ENA

Glycine max cultivar:Williams i mutation, PI 691662

Project description:Glycine max cultivar:Williams i mutation, PI 691662 Genome sequencing

| PRJNA988802 | ENA

Glycine max cultivar:Maple Arrow (PI 548593)

Project description:Glycine max cultivar:Maple Arrow (PI 548593) Genome sequencing and assembly

| PRJNA835489 | ENA

Transcription profiling of mouse splenic B cells expressing a dominant-negative p300 allele which lacks histone acetyltransferase activity

Project description:The p300 protein is one of more than 15 known mammalian HATs. We have used mice that carry a mutant allele of the p300 gene, which encodes a full-length protein that, however, lacks detectable acetyltransferase (AT)-activity and that acts in a dominant-negative manner. In addition, the allele is conditional, so that it can be expressed in a tissue-specific manner with the help of mice that express Cre-recombinase. Expression of the mutant allele specifically in B-cells, using CD19-cre mice, results in premature death with full penetrance from an autoimmune disease that closely mimics systemic lupus erythematosus (SLE) in its pathological manifestations.

2006-07-01 | E-MEXP-747 | biostudies-arrayexpress

Allele-Specific gene editing approach for a dominant form of Epidermolysis Bullosa Simplex

Project description:Allele-Specific gene editing approach for a dominant form of Epidermolysis Bullosa Simplex

| PRJNA1032582 | ENA

Sorghum bicolor cultivar:PI 508366

Project description:Sorghum bicolor cultivar:PI 508366 Genome sequencing

| PRJNA337062 | ENA