Project description:Expression profiles of HeLa CD4+ cells transfected with epitope-tagged eTat plasmid, or parental plasmid pCep4. Cells are unsynchronized and untreated.
Project description:Expression profiles of HeLa CD4+ cells transfected with epitope-tagged eTat plasmid, or parental plasmid pCep4. Cells are unsynchronized and untreated. Keywords: other
Project description:Expression profiles of HeLa CD4+ cells transfected with epitope-tagged eTat plasmid. Cells are treated with nocodazole and hydroxyurea. Keywords: time-course
Project description:Expression profiles of HeLa CD4+ cells transfected with epitope-tagged eTat plasmid. Cells are treated with nocodazole and hydroxyurea.
Project description:The distribution of histone variants H2Abbd and macroH2A in 13 regions of the HG18 assembly have been studied using a variant of the ChIP-on-Chip technique. HeLa S3 cell lines expressing tagged histones H2A, H2Abbd or macroHA were obtained using retroviral transfer. DNA fractions associated with tagged histones were isolated using a two-step purification procedure that involved affinity chromatography on a column with anti-FLAG antibodies, followed by affinity chromatography on a Ni-agarose column. The obtained genomic DNA samples were analyzed by hybridization with custom NimbleGene genomic microarrays. Two samples. Test sample 1 is HeLa S3 cells expressing epitope-tagged histone H2Abbd and test sample 2 is HeLa S3 cells expressing epitope-tagged histone macroH2A . The control for both test sample 1 and test sample 2 is HeLa S3 expressing epitope-tagged histone H2A. Two copies of each probe per array were made.
Project description:Expression profiles of HeLa CD4+ cells transfected with parental vector pCep4. Cells are treated with nocodazole and hydroxyurea. Keywords: time-course
Project description:Transforming growth factor β (TGFβ) signaling pathways are integral for a plethora of biological processes. SMAD2 and SMAD3 are the principal transcriptional effectors of TGFβ superfamily ligands, yet quantitative, genome-wide mapping of their DNA-associated complexes under physiological contexts has remained limited due to the lack of specific, robust models. Here, we generated two versatile epitope-tagged mouse models in which endogenous SMAD2 and SMAD3 proteins are globally tagged with HA and PA, respectively. To demonstrate the broad application of our models, we evaluated our lines in ovarian biology. By integrating genomic and transcriptomic analyses, we identified direct genes induced by the GDF9-SMAD2/3 axis and discovered gene sets suppressed by this signaling cascade, highlighting a previously underappreciated role of GDF9 in attenuating competing pathways to ensure proper ovarian granulosa cell fate transitions. Together, these epitope-tagged SMAD mouse models provide extensive, applicable in vivo genetic toolkits for tissue-specific dissection of TGFβ family signaling.