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ZBTB48 is a pioneer factor regulating B-cell-specific CIITA expression [RNA-seq]

ABSTRACT: ZBTB48 is a pioneer factor regulating B-cell-specific CIITA expression [RNA-seq]

PROVIDER: PRJNA997299 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25387100_1.fastq.gz	Fastqsanger.gz
	SRR25387100_2.fastq.gz	Fastqsanger.gz
	SRR25387101_1.fastq.gz	Fastqsanger.gz
	SRR25387101_2.fastq.gz	Fastqsanger.gz
	SRR25387102_1.fastq.gz	Fastqsanger.gz

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ZBTB48 is a pioneer factor regulating B-cell-specific CIITA expression

Project description:CIITA is the master regulator of MHC II genes expression and hence the adaptive immune response. CIITA expression itself is tightly regulated by three cell type-specific promoters, pI, pIII, and by pIV, and can also be induced by IFNg in non-immune cells. While key regulatory elements have been identified within these promoters, knowledge of transcription factors regulating CIITA is incomplete. Here, we demonstrate that the telomere-binding protein and transcriptional activator ZBTB48 directly binds to both critical activating elements within CIITA pIII and is essential for its gene expression. ZBTB48 establishes open chromatin at CIITA pIII upstream of activating H3K4me3 modifications both priming CIITA transcription for IFNg-induction and ensuring constitutive expression in primary murine B cells. Hence, ZBTB48 acts as a molecular on-off-switch for B-cell-specific CIITA expression.

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ZBTB48 is a pioneer factor regulating B-cell-specific CIITA expression.

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ZBTB48 is a pioneer factor regulating B-cell-specific CIITA expression [RNA-seq]

Project description:CIITA is the master regulator of MHC II gene expression and hence the adaptive immune response. CIITA expression itself is tightly regulated by three cell type-specific promoters, pI, pIII, and by pIV. Here, we demonstrate that the telomere-binding protein and transcriptional activator ZBTB48 directly binds to both the critical activating elements within CIITA pIII and is essential for its gene expression. ZBTB48 establishes open chromatin at CIITA pIII upstream of activating H3K4me3 modifications both priming CIITA transcription for IFNg-induction and ensuring constitutive expression in primary murine B cells. Hence, ZBTB48 acts as a molecular on-off-switch for B-cell-specific CIITA expression.

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ZBTB48 is a pioneer factor regulating B-cell-specific CIITA expression [FAIRE-seq]

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ZBTB48 is a pioneer factor regulating B-cell-specific CIITA expression [ChIP-seq]

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ZBTB48 is a pioneer factor regulating B-cell-specific CIITA expression [ChIP-seq]

Project description:ZBTB48 is a pioneer factor regulating B-cell-specific CIITA expression [ChIP-seq]

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ZBTB48 is a pioneer factor regulating B-cell-specific CIITA expression [FAIRE-seq]

Project description:ZBTB48 is a pioneer factor regulating B-cell-specific CIITA expression [FAIRE-seq]

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ZBTB48 is a pioneer factor regulating B-cell-specific CIITA expression.

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-12-26 | GSE237959 | GEO

ZBTB48 is a priming factor regulating B-cell-specific CIITA expression

Project description:The class-II transactivator (CIITA) is the master regulator of MHC class-II gene expression and hence the adaptive immune response. Three cell type-specific promoters (pI, pIII, and pIV) are involved in the regulation of CIITA expression, which can be induced by IFN-γ in non-immune cells. While key regulatory elements have been identified within these promoters, our understanding of the transcription factors regulating CIITA expression is incomplete. Here, we demonstrate that the telomere-binding protein and transcriptional activator ZBTB48 directly binds to both critical activating elements within the B-cell-specific promoter CIITA pIII. ZBTB48 knockout impedes the CIITA/MHC-II expression program induced in non-APC cells by IFN-γ, and loss of ZBTB48 in mice silences MHC-II expression in pro-B and immature B-cells. Transcriptional regulation of CIITA by ZBTB48 is enabled by ZBTB48-dependent chromatin opening at CIITA pIII upstream of activating H3K4me3 marks. We conclude that ZBTB48 primes CIITA pIII by acting as a molecular on-off switch for B-cell-specific CIITA expression.

| S-SCDT-10_1038-S44318-024-00306-Y | biostudies-other

CIITA

Project description:CIITA

| PRJEB5958 | ENA