Opisthorchis felineus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA997720 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Opisthorchis felineus de novo transcriptome sequencing
| PRJNA257351 | ENA
Project description:Opisthorchis felineus Genome sequencing and assembly
| PRJNA413383 | ENA
Project description:Opisthorchis felineus is one of the three most medically important species belonging to the family of fish-borne zoonotic trematodes known as Opisthorchiidae. O. felineus is endemic to the river plains of Western Siberia and Eastern Europe and it is estimated that more than 1.6 million people could be infected with this parasite. To aid in the development of better control and diagnosis strategies for opisthorchiasis, we set out to deduce the secreted proteome of O. felineus. Adult flukes were collected from experimentally infected hamsters and cultured in vitro in serum-free media. We extracted proteins from different compartments of the O. felineus secretome, including (i) soluble excretory/secretory (ES) products; (ii) secreted microvesicles (MVs); and (iii) tegument. The tegument was further separated into three fractions of varying solubility via sequential extraction.
2023-09-29 | PXD045798 | Pride
Project description:Opisthorchis felineus is one of the three most medically important species belonging to the family of fish-borne zoonotic trematodes known as Opisthorchiidae. O. felineus is endemic to the river plains of Western Siberia and Eastern Europe and it is estimated that more than 1.6 million people could be infected with this parasite. To aid in the development of better control and diagnosis strategies for opisthorchiasis, we set out to deduce the secreted proteome of O. felineus. Adult flukes were collected from experimentally infected hamsters and cultured in vitro in serum-free media. We extracted proteins from different compartments of the O. felineus secretome, including (i) soluble excretory/secretory (ES) products; (ii) secreted microvesicles (MVs); and (iii) tegument. The tegument was further separated into three fractions of varying solubility via sequential extraction.
2023-02-24 | PXD040364 | Pride
Project description:A significant number of pathological conditions, accompanied by chronic non-healing wounds, demands searching for new modern therapeutic approaches. Well-documented ability of O. felineus to initiate extracellular matrix (ECM) remodeling and liver epithelium regeneration suggests that its bioactive molecules may stimulate skin wound healing processes. The aim of this study was to investigate the wound healing potential of the Opisthorchis felineus excretory-secretory and lysate proteins on a murine model. The following methods were used for the study: histological (wounded skin condition), immunohistochemical (ECM, neoangiogenesis, O. felineus GST and TPx proteins), gene expression analysis (inflammation, angiogenesis, ECM condition). O. felineus excretory-secretory product (ESP) and lysate proteins have revealed wound-healing potential, they: i) reduce inflammation levels, ii) modulate vascular response, iii) stimulate collagen deposition and dermal ECM remodeling. Additional proteomic analysis of adult O. felineus ESP and lysate samples was conducted. Proteomic analysis approach called GeLC-MS / MS was chosen to study of the excretory-secretory product and lysate proteins. This approach is based on one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), in-gel protein digestion with trypsin followed by liquid chromatography-tandem mass spectrometry. The SDS-PAGE step allow to removes hemozoin also as detergents, buffers and salts from the protein extract that may interfere with mass spectrometry analysis
2023-03-11 | PXD037991 | Pride
Project description:Studing of human biliary microbiota in case of Opisthorchis felineus infection
| PRJEB12755 | ENA
Project description:The proteomic content of the extracellular vesicles (EVs) released by the liver fluke Opisthorchis viverrini has been addressed in the past, but they have focused on the small population pelleting at 120k vesicles (also named exosomes). Here we provide the first proteomic analysis of vesicles pelleting at 15k (microvesicles) using LC-MS/MS.
2024-09-20 | PXD020356 | Pride
Project description:The proteomic content of the extracellular vesicles (EVs) released by the liver fluke Opisthorchis viverrini has been addressed in the past. Here we employ a more comprehensive purification method of the 120k subpopulation of EVs and analyze proteins present in different locations of these EVs (including external trypsin-liberated peptides, cargo proteins and membrane proteins) using LC-MS/MS.
2022-03-15 | PXD020345 | Pride