Project description:The main objective of this study is to analyze changes in whole-genome transcriptomic profile of ERDMAN_2020(-) strain of Mycobacterium tuberculosis Erdman after ATc-dependent conditional depletion of ERDMAN_2020 by RNA Seq. Total 798 transcripts were found differentially regulated in three biological replicates with log2 fold change ≥ 1 and p-value ≤ 0.05 from. ERDMAN_2020 belongs to MerR family of transcription regulators. Treatment of ERDMAN_2020(-) strain with 50ng/ml ATc for 4 days resulted in accumulation of 338 transcripts and suppression of 460 transcripts, compared to the empty vector control.

2023-07-19 | GSE214729 | GEO

Transcriptome analysis of VapC21 overexpression and deletion in Mycobacterium tuberculosis.

Project description:We report the effects of VapC21 overexpression on Mycobacterium tuberculosis H37Rv strain. The total RNA was isolated from M. tuberculosis harboring either pTetR-int or pTetR-Int-vapC21 the expression was induced with 50 ng/ml anhydrotetracycline for 24 hrs. We report the effect of deletion VapC21 on the transciptional profile of Mycobacterium tuberculosis Erdman strain. For RNA-seq analysis, total RNA was isolated from mid-log phase culture of either parental or △vapC21 mutant strain.

2020-10-05 | GSE154320 | GEO

Mycobacterium tuberculosis

Project description:Mycobacterium tuberculosis Raw sequence reads

| PRJNA884747 | ENA

Mycobacterium tuberculosis

Project description:Mycobacterium tuberculosis Raw sequence reads

| PRJNA302362 | ENA

Mycobacterium tuberculosis

Project description:Mycobacterium tuberculosis Raw sequence reads

| PRJNA480117 | ENA

Mycobacterium tuberculosis

Project description:Mycobacterium tuberculosis Raw sequence reads