Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: RNA-Seq of importin alpha2 silenced Nicotiana benthamiana

PROVIDER: PRJNA998578 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25422550_1.fastq.gz	Fastqsanger.gz
	SRR25422550_2.fastq.gz	Fastqsanger.gz
	SRR25422551_1.fastq.gz	Fastqsanger.gz
	SRR25422551_2.fastq.gz	Fastqsanger.gz
	SRR25422552_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

Project description:Small RNA-Seq of Bamboo mosaic virus infected leaves of importin alpha2 silenced Nicotiana benthamiana

| PRJNA1027972 | ENA

HeLa cells: overexpression of importin alpha2

Project description:To test whether nuclear importin alpha2 can regulate transcription, we sought to examine gene expression changes in cells with nuclear accumulation of importin alpha2 by performing microarray analysis. We designed an experiment in which EGFP-fused full-length importin alpha2 was transfected into HeLa cells. To exclude the possibility that exogenous, full-length importin alpha2 protein enhances nuclear transport of karyophilic proteins such as transcription factors and thereby directly influences gene expression, a mutant of importin alpha2 which is mutated in the C-terminal CAS-binding domain was also transfected, so that it is never recycled to the cytoplasm and shows complete nuclear localization. We undertook to investigate whether there were changes in gene expression common to cells expressing the full-length importin alpha2 and the C-terminal mutant (C-mutant) isoform. EGFP vs. EGFP-importin alpha2 full length, or EGFP vs EGFP-importin alpha2 CAS-binding mutant. One replicate each. EGFP-expressing cells are used as a control.

2010-11-24 | E-GEOD-25303 | biostudies-arrayexpress

HeLa cells: overexpression of importin alpha2

2010-11-24 | GSE25303 | GEO

Small-RNA sequencing of WUS-induced GFP RNA degradation in Nicotiana benthamiana leaves

Project description:The 35S::GFP fluorescence was silenced 6-day after infiltration to Nicotiana benthamiana leaves due to the post-transcriptional gene silencing, but became stable by adding the viral suppressor 2b which repressed RNA silencing in plants. We performed the small RNA high throughput sequencing to test whether WUS can inhibit 2b functions in repressing plant RNA silencing.

2021-02-22 | GSE128863 | GEO

Identification of NMD regulated genes in Nicotiana benthamiana

Project description:Nonsense mediated decay (NMD) is a translation termination coupled quality control system. NMD identifies and degrades aberrant mRNAs containing premature termination codons, thereby preventing the accumulation of detrimental truncated proteins. NMD also controls the expression of several normal mRNAs and non-coding transcripts. RNA-seq assays were conducted to identify the NMD regulated genes in Arabidopsis. However, NMD targets have not been studied in other plants. To identify the conserved NMD targets, we wanted to identify the NMD regulated genes in Nicotiana benthamiana model species. Virus induced gene silencing was used to inactivate UPF1 NMD key factor in N. benthamiana, and then comparative RNA-seq experiment was carried out using UPF1-silenced and control–silenced plants. We found that in N. benthamiana significantly more genes are controlled by NMD than in Arabidopsis and that, surprisingly few conserved NMD targets can be recognized. These results suggest that while the mechanism of NMD is highly conserved, the set of NMD controlled genes can be quickly changed in plants.

2019-07-01 | GSE109130 | GEO

Tobacco RNA Dependent RNA Polymerase 1 affects the expression of defence related genes in Nicotiana benthamiana during geminivirus infection

Project description:RNA dependent RNA Polymerase 1 of Nicotiana tabacum modulates ToLCV pathogenesis by influencing a number of defence related genes in N. benthamiana plants.

2020-06-03 | GSE144074 | GEO

NGS data of Nicotiana benthamiana 16c plants after CRISPR/Cas9 gene editing

Project description:Provided data came from a detailed study on Nicotiana benthamiana 16c plants where we use Tobacco Rattle Virus (TRV) as a molecular switch to change the chromatin state of a reporter gene (P35S::GFP) from an actively transcribed to a transcriptionally silenced state. Our approach enables us to interrogate different chromatin states of the same locus with the same set of CRISPR/Cas9 genome editing reagents and systematically describe the effect of chromatin state on the frequency and type of mutations induced at various Cas9 targets in a huge set of independently edited cells.

2022-05-04 | E-MTAB-10256 | biostudies-arrayexpress

Solanaceae comparative gene expression profiling

Project description:Six different Solanaceae species, Potato (Solanum tuberosum), Tomato (Lycopersicum esculentum), Pepper (Capsicum annuum), Tobacco (Nicotiana tabacum), Petunia and Nicotiana benthamiana were grown at 25C, 16h light and 8h darkness. Mature leaves were harvested after 4-6 weeks. RNA was isolated using Qiagen RNeasy. Tomato, pepper, petunia tobacco and N. benthamiana samples were hybridized against potato samples. Keywords: Direct comaprison

2007-09-01 | GSE8234 | GEO

Project description:RNA-Seq of Nicotiana benthamiana

| PRJNA778182 | ENA

Homo sapiens

Project description:HeLa cells: overexpression of importin alpha2

| PRJNA134789 | ENA