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Gene expression profiles of HYDROX-cultured human liver organoids compared to those of Matrigel-cultured human liver organoids.

ABSTRACT: Gene expression profiles of HYDROX-cultured human liver organoids compared to those of Matrigel-cultured human liver organoids.

PROVIDER: PRJNA998921 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25432266_1.fastq.gz	Fastqsanger.gz
	SRR25432266_2.fastq.gz	Fastqsanger.gz
	SRR25432267_1.fastq.gz	Fastqsanger.gz
	SRR25432267_2.fastq.gz	Fastqsanger.gz
	SRR25432268_1.fastq.gz	Fastqsanger.gz

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Gene expression profiles of HYDROX-cultured human liver organoids compared to those of Matrigel-cultured human liver organoids.

Project description:Human liver organoids, an in vitro 3D culture system to recapitulate biological tissue, are expected to be used for drug discovery. However, Matrigel, the most widely used extracellular matrix for organoid culture, has concerns about safety and reproducibility since it is murine-derived. Morever, low hepatic functions of human liver organoids compared to primary human hepatocytes is considered a challenge. Herein, we attempted to culture human liver organoids, established from primary (cryopreserved) human hepatocytes (PHH), using HYDROX, a chemically defined 3D nanofiber. While proliferative capacity of human liver organoids was lost by HYDROX-culture, the gene expression level of a hepatocyte marker CYP3A4 and the CYP3A4 metabolic activity in HYDROX-cultured liver organoids were significantly improved, comparable to those of PHH. HYDROX-cultured liver organoids when treated with hepatotoxic drugs such as acetaminophen showed similar cell viability to that of PHH, suggesting that HYDROX-cultured liver organoids could be applied to drug-induced hepatotoxicity test. Furthermore, HYDROX-cultured liver organoids maintained its functions for up to 35 days and could be used to estimate chronic drug-induced hepatotoxicity such as those of fialuridine. Our findings demonstrated that human liver organoids obtained high liver functions by HYDROX-culture, meaning that HYDROX could contribute to drug discovery as a novel biomaterial.

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Whole transcriptome data of human liver organoids cultured in Matrigel and polyisocyanopeptides-based hydrogel

Project description:End-stage liver diseases are an increasing health burden and liver transplantations are currently the only curative treatment option. Due to a lack of donor livers, alternative treatments are urgently needed. Human liver organoids are very promising for regenerative medicine, however, organoids are currently cultured in Matrigel, which is extracted from the extracellular matrix of the Engelbreth-Holm-Swarm mouse sarcoma. Matrigel is poorly defined, suffers from high batch-to-batch variability and is of murine origin, which limits clinical application of organoids. Here, a novel hydrogel based on polyisocyanopeptides (PIC) and laminin-111 is described for human liver organoid culture. PIC is a synthetic hydrogel with thermodynamic properties, making it easy to handle and very attractive for clinical applications. Organoids in an optimized PIC hydrogel proliferate at rates comparable to Matrigel; proliferation rates are stiffness-dependent, with lower stiffnesses being optimal for organoid proliferation. Moreover, organoids can be efficiently differentiated towards hepatocyte-like cells with key liver functions. This proliferation and differentiation potential can be maintained over at least 16 passages. Our results indicate that PIC is a promising material for human liver organoid culture and has the potential to be used in a variety of clinical applications including cell therapy and tissue engineering.

2020-12-23 | GSE143223 | GEO

Tissue extracellular matrix hydrogels as alternative to Matrigel for culturing gastrointestinal organoids

Project description:To determine the performance of the decellularized GI tissue ECM hydrogel, we analyzed differences in mRNA expression levels in native gastric tissues, gastric organoids cultured in Matrigel and those cultured in decellularized gastric ECM hydrogel. We also compared mRNA expression levels of native small intestinal tissues, small intestinal organoids grown in Matrigel and those grown in decellularized intestinal ECM hydrogel.

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Project description:Investigate the functional capabilities of human iPSC-derived liver organoids generated on Matrigel or self-assembed in rotating wall vessel (RWV) via bulk RNA-seq, RT-qPCR and immunostaining, to provide a simple and high-throughput way to generate Matrigel-free liver organoids for research and clinical applications

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Whole transcriptome data of human liver organoids cultured in Matrigel and polyisocyanopeptides-based hydrogel

Project description:Whole transcriptome data of human liver organoids cultured in Matrigel and polyisocyanopeptides-based hydrogel

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Tissue extracellular matrix hydrogels as alternative to Matrigel for culturing gastrointestinal organoids

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RNA-Seq of human spinal cord organoids generated in Matrigel and alginate

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