Project description:We decreased TAL1 and NKX3.1 proteins levels using lentiviral deliveries of shTAL1 and shNKX3.1 in TAL1 expressing human T-ALL cell lines. Growth curves of cell lines showed that reduced TAL1 and NKX3.1 expressions resulted in a first phase where the number of cells did not increase likewise the shCTL expressing cells. To identify TAL1 and NKX3.1 target genes involved in T-ALL cell growth, we performed gene expression profiling of cell lines expressing shTAL1, shNKX3.1 or shCTL after two and three days of culture. <br><br><br><br>
Project description:Mouse embryonic fibroblasts (MEF) were isolated via enzymatic dissociation of 3 E14.5 embrya bearing epiblast specific inactivation of the murine Elavl1 locus as 3 littermate controls. Individual Fibroblast cultures were maintained in complete DMEM medium containing 10%FBS at 370C with 5%CO2. Cultures were passaged for 3 times and harvested for RNA isolation when they reached 60-70% conflunecy.
Project description:Comparison of gene expression profiles in CD133+ Vs alpha2integrin low cell populations from patients with prostate cancer versus patients with benign prostatic hyperplasia