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Novel cell lines from mouse epiblast share defining features with human embryonic stem cells

ABSTRACT: Novel cell lines from mouse epiblast share defining features with human embryonic stem cells

OTHER RELATED OMICS DATASETS IN: GSE7902

PROVIDER: PRJNA99953 | ENA |

REPOSITORIES: ENA

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Novel cell lines from mouse epiblast share defining features with human embryonic stem cells

Project description:The application of human embryonic stem (ES) cells has an inherent reliance on understanding the starting cell population. Human ES cells differ from mouse ES cells and the specific embryonic origin of both cell types is unclear. Previous work suggested that mouse ES cells could only be obtained from the embryo prior to implantation in the uterus. Here we show that cell lines can be derived from the epiblast, a tissue of the post-implantation embryo that generates the embryo proper. These cells, which we refer to as EpiSCs (post-implantation epiblast-derived stem cells), express transcription factors known to regulate pluripotency, maintain their genomic integrity, and robustly differentiate into the major somatic cell types as well as primordial germ cells (PGCs). The post-ES cell lines are distinct from mouse ES cells in their epigenetic state and the signals controlling their differentiation. Furthermore, post-ES and human ES cells share patterns of gene expression and signalling responses that normally function in the epiblast. These results show that epiblast cells can be maintained as stable cell lines and interrogated to understand how pluripotent cells generate distinct fates during early development. This SuperSeries is composed of the SubSeries listed below.

2007-06-27 | GSE7902 | GEO

Isolation of epiblast stem cells from pre-implantation mouse embryos

Project description:Pluripotent stem cells provide a platform to interrogate control elements that function to generate all cell types of the body. Despite their utility for modeling development and disease, the relationship of mouse and human pluripotent stem cell states to one another remains largely undefined. We have shown that mouse embryonic stem (ES) cells and epiblast stem cells (EpiSCs) are distinct, pluripotent states isolated from pre- and post-implantation embryos respectively. Human ES cells are different than mouse ES cells and share defining features with EpiSCs, yet are derived from pre-implantation human embryos. Here we show that EpiSCs can be routinely derived from pre-implantation mouse embryos. The pre-implantation-derived EpiSCs exhibit molecular features and functional properties consistent with bona fide EpiSCs. These results provide a simple method for isolating EpiSCs and offer direct insight into the intrinsic and extrinsic mechanisms that regulate the acquisition of distinct pluripotent states. 6 total samples were analyzed. Three pluripotent cell types (mES cells, E3.5 EpiSCs, and E5.5 EpiSCs) were compared with and without treatment of SB431542 for 4 days.

2011-01-25 | E-GEOD-26814 | biostudies-arrayexpress

Formative pluripotent stem cells show features of epiblast cells poised for gastrulation

Project description:The pluripotency of mammalian early and late epiblast could be recapitulated by naïve embryonic stem cells (ESCs) and primed epiblast stem cells (EpiSCs), respectively. However, these two states of pluripotency may not be sufficient to reflect the full complexity and developmental potency of the epiblast during mammalian early development. Here we report the establishment of self-renewing formative pluripotent stem cells (fPSCs) which manifest features of epiblast cells poised for gastrulation. fPSCs can be established from different mouse ESCs, pre-/early-gastrula epiblasts and induced PSCs. Similar to pre-/early-gastrula epiblasts, fPSCs show the transcriptomic features of formative pluripotency, which are distinct from naïve ESCs and primed EpiSCs. fPSCs show the unique epigenetic states of E6.5 epiblast, including the super-bivalency of a large set of developmental genes. Just like epiblast cells immediately before gastrulation, fPSCs can efficiently differentiate into three germ layers and primordial germ cells (PGCs) in vitro. Thus, fPSCs highlight the feasibility of using PSCs to explore the development of mammalian epiblast.

2021-02-19 | GSE154290 | GEO

Isolation of epiblast stem cells from pre-implantation mouse embryos

2011-01-25 | GSE26814 | GEO

Pancreatic adenocarcinoma human organoids share structural and genetic features with primary tumors

Project description:Pancreatic adenocarcinoma human organoids share structural and genetic features with primary tumors

| PRJNA498740 | ENA

Pancreatic adenocarcinoma human organoids share structural and genetic features with primary tumors

Project description:Pancreatic adenocarcinoma human organoids share structural and genetic features with primary tumors

| PRJNA498741 | ENA

Epiblast stem cell subpopulations represent mouse embryos of distinct pregastrulation stages

Project description:Embryonic stem cells (ESCs) comprise at least two populations of cells with divergent states of pluripotency. Here, we show that epiblast stem cells (EpiSCs) also comprise two distinct cell populations that can be distinguished by the expression of a specific Oct4-GFP marker. These two subpopulations, Oct4-GFP positive and negative EpiSCs, are capable of converting into each other in vitro. Oct4-GFP positive and negative EpiSCs are distinct from ESCs with respect to global gene expression pattern, epigenetic profile, and Oct4 enhancer utilization. Oct4-GFP negative cells share features with cells of the late mouse epiblast and cannot form chimeras. However, Oct4-GFP positive EpiSCs, which only represent a minor EpiSC fraction, resemble cells of the early epiblast and can readily contribute to chimeras. Our findings suggest that the rare ability of EpiSCs to contribute to chimeras is due to the presence of the minor EpiSC fraction representing the early epiblast.

2011-02-24 | GSE17984 | GEO

Formative pluripotent stem cells show features of epiblast cells poised for gastrulation

Project description:Formative pluripotent stem cells show features of epiblast cells poised for gastrulation

| PRJNA645812 | ENA

Peripheral nerve resident macrophages share tissue-specific programming and features of activated microglia

Project description:Peripheral nerve resident macrophages share tissue-specific programming and features of activated microglia

| PRJNA610789 | ENA

FOHM data share

Project description:FOHM data share

| PRJEB28249 | ENA