Project description:We developed an optimized, low-cost, split-pool barcoding-based multimodal profiling protocol based upon SHARE-seq (concurrent single-cell ATAC/RNA-seq). With SHARE-seq, we profiled human kidney samples from multiple different anatomical regions. Therefore, we develop a large-scale multimodal single-cell atlas for 3D anatomy of the human kidney.
Project description:A growing body of single-cell multi-omic tools enables a better understanding of gene regulation underlying development and disease. Tools mapping chromatin and gene expression (such as SHARE-seq) are limited in sensitivity and scalability. Here, we describe SHARE-seqV2 and its associated cloud-computing resource, enabling single-cell analysis at improved quality, robustness, and scale. We apply SHARE-seqV2 across various cell lines, primary tissues, and immune cells, demonstrating a generalizable platform for single-cell multi-omics.
Project description:The growing smartphone penetration and the integration of smartphones into people's everyday practices offer researchers opportunities to augment survey measurement with smartphone-sensor measurement or to replace self-reports. Potential benefits include lower measurement error, a widening of research questions, collection of in situ data, and a lowered respondent burden. However, privacy considerations and other concerns may lead to nonparticipation. To date, little is known about the mechanisms of willingness to share sensor data by the general population, and no evidence is available concerning the stability of willingness. The present study focuses on survey respondents' willingness to share data collected using smartphone sensors (GPS, camera, and wearables) in a probability-based online panel of the general population of the Netherlands. A randomized experiment varied study sponsor, framing of the request, the emphasis on control over the data collection process, and assurance of privacy and confidentiality. Respondents were asked repeatedly about their willingness to share the data collected using smartphone sensors, with varying periods before the second request. Willingness to participate in sensor-based data collection varied by the type of sensor, study sponsor, order of the request, respondent's familiarity with the device, previous experience with participating in research involving smartphone sensors, and privacy concerns. Willingness increased when respondents were asked repeatedly and varied by sensor and task. The timing of the repeated request, one month or six months after the initial request, did not have a significant effect on willingness.