Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Manipulating the 3D organization of the largest synthetic yeast chromosome [HiC]

ABSTRACT: Manipulating the 3D organization of the largest synthetic yeast chromosome [HiC]

PROVIDER: PRJNA999664 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25448732_1.fastq.gz	Fastqsanger.gz
	SRR25448732_2.fastq.gz	Fastqsanger.gz
	SRR25448733_1.fastq.gz	Fastqsanger.gz
	SRR25448733_2.fastq.gz	Fastqsanger.gz
	SRR25448734_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Manipulating the 3D organization of the largest synthetic yeast chromosome

Project description:Manipulating the 3D organization of the largest synthetic yeast chromosome

| PRJNA999662 | ENA

Manipulating the 3D organization of the largest synthetic yeast chromosome [WGS]

Project description:Manipulating the 3D organization of the largest synthetic yeast chromosome [WGS]

| PRJNA999665 | ENA

Manipulating the 3D organization of the largest synthetic yeast chromosome [RNAseq]

Project description:Manipulating the 3D organization of the largest synthetic yeast chromosome [RNAseq]

| PRJNA999663 | ENA

Manipulating the 3D organization of the largest synthetic yeast chromosome [HiC]

Project description:Whether synthetic genomescan power life has attracted broad interest in the synthetic biology field, especially when the synthetic genomes are extensively modified with thousands of designer features. Here we reportde novosynthesis of the largest eukaryotic chromosome thus far, synIV, a 1,454,621-bpSaccharomyces cerevisiaechromosome resulting from extensive genome streamlining and modification. During the construction ofsynIV, we developed megachunk assembly combined with a hierarchical integration strategy, which significantly increased the accuracy and flexibility of synthetic chromosome construction and facilitated chromosome debugging. In addition to the drastic sequence changes made to synIV by rewriting it, we further manipulated the three-dimensional structure of synIV in the yeast nucleus to explore spatial gene regulation within the nuclear space. Surprisingly, we found few gene expression changes, suggesting that positioning inside the yeast nucleoplasm plays a minor role in gene regulation. Lastly, we tethered synIV to the inner nuclear membrane via its hundreds of loxPsym sites and observed transcriptional repression of the entire chromosome, demonstrating chromosome-wide transcription manipulation without changing the DNA sequences. Our manipulation of the spatial structure of the largest synthetic yeast chromosome shed light on higher-order architectural design of the synthetic genomes.

2023-08-04 | GSE239532 | GEO

Manipulating the 3D organization of the largest synthetic yeast chromosome [WGS]

2023-08-04 | GSE239535 | GEO

Manipulating the 3D organization of the largest synthetic yeast chromosome [RNAseq]

2023-08-04 | GSE239534 | GEO

Manipulating the 3D organization of the largest synthetic yeast chromosome

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-08-04 | GSE239536 | GEO

Spen regulates X chromosome inactivation (HiC in NPC)

Project description:HiC on Spen-degron TX1072 clones of neural progenitor cells (NPCs). HiC in 2 biological replicates before and after. 24h of auxin treatment.

2019-12-12 | GSE140388 | GEO

Single-cell DNA replication profiling identifies spatiotemporal developmental dynamics of chromosome organization [HiC-seq]

Project description:Single-cell DNA replication profiling identifies spatiotemporal developmental dynamics of chromosome organization [HiC-seq]

| PRJNA454725 | ENA

Anti-cancer drugs curaxins target the 3D genome organization [HiC]

Project description:Anti-cancer drugs curaxins target the 3D genome organization [HiC]

| PRJNA482566 | ENA