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TranscriptomicsIntroducing synthetic thermostable RNase inhibitors in single-cell RNA-seq (Bulk RNA-seq libraries)
Single-cell RNA-sequencing (scRNAseq) is revolutionizing biomedicine, propelled by advances in methodology, ease of use, and cost reduction of library preparation. Over the past decade, there have been remarkable technical improvements in most aspects of single-cell transcriptomics. Yet, there has be...
ORGANISM(S): Mus musculus 
2024-07-26 | E-MTAB-13899 | biostudies-arrayexpress
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TranscriptomicsMulti-modal single-cell sequencing of differentiating mESCs using Smart-seq3 and scATAC-seq
We report dynamics of X-chromosome upregulation (XCU) along X-chromosome inactivation (XCI) in mESCs as they differentiate into EpiSCs. F1 hybrid C57BL6/J × CAST/EiJ male and female mESCs were grown in serum/LIF conditions were differentiated using Fgf2 and Activin A for 1, 2, 4 and 7 days to induce ...
ORGANISM(S): Mus musculus 
2022-02-14 | E-MTAB-10714 | biostudies-arrayexpress
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TranscriptomicsIntroducing synthetic thermostable RNase inhibitors in single-cell RNA-seq
Single-cell RNA-sequencing (scRNAseq) is revolutionizing biomedicine, propelled by advances in methodology, ease of use, and cost reduction of library preparation. Over the past decade, there have been remarkable technical improvements in most aspects of single-cell transcriptomics. Yet, there has be...
ORGANISM(S): Mus musculus 
2024-07-29 | E-MTAB-13873 | biostudies-arrayexpress
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TranscriptomicsAllelic dilution series series of C57BL6/J:CAST/EiJ RNA using Smart-seq3
Technical control for allelic detection using Smart-seq3. Liver RNA from pure C57BL6/J and CAST/EiJ strains was combined at varying ratios (0:1, 1:7, 1:3, 3:5, 1:1, 5:3, 3:1, 7:1, 1:0) for a total of 200 pg RNA per sample.
ORGANISM(S): Mus musculus 
2022-02-14 | E-MTAB-10709 | biostudies-arrayexpress
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TranscriptomicsSingle-cell RNA-Seq reveals dynamic, random monoallelic gene expression in mammalian cells
...me in two copies, which can have different DNA sequence and where one is maternal and one is paternal. In a particular cell, a gene could potentially be expressed from both copies (biallelic expression) or only one (monoallelic). We performed RNA-Sequencing on individual cells, from zygote to the cel...
ORGANISM(S): Mus musculus 
2014-01-10 | E-GEOD-45719 | biostudies-arrayexpress
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TranscriptomicsTranscription profiling of occipital cortex tissue from male and female humans, macaques and marmosets to investigate sex differences in primate brain
We compared gene expression patterns between the occipital cortex tissues of four male and four female individuals in three species: an ape (human, Homo sapiens), an Old World monkey (macaque; Macaca fascicularis), and a New World monkey (marmoset; Callithrix jacchus). To do so, we hybridized cDNA fr...
ORGANISM(S): Callithrix jacchus 
2008-03-01 | E-MEXP-1182 | biostudies-arrayexpress
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GenomicsWhole-genome sequencing of SARS-CoV-2 samples in Sweden during early 2022
We developed an extraction-free qPCR assay to identify Omicron BA.1 cases and verified lineage by sequencing. We find that BA.2 variants show almost twice the viral load (Ct) compared to both BA.1 as well as Delta variants.
ORGANISM(S): Severe acute respiratory syndrome coronavirus 2 
2022-05-25 | E-MTAB-11650 | biostudies-arrayexpress
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TranscriptomicsSingle-cell RNA-sequencing of mouse periaqueductal gray (PAG) neurons using Smart-seq2.
We optimized Voltage-Seq which combines, all-optical physiology, spatial mapping, on-site classification, and RNA-transcriptomics to robustly increase the throughput of synaptic connectivity testing and targeted molecular classification of postsynaptic neurons. Single-cell RNA-sequencing was performe...
ORGANISM(S): Mus musculus 
2023-06-22 | E-MTAB-13104 | biostudies-arrayexpress
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